Enhancement of the Receptor Binding and Nb2 Proliferation Activities of Rat Prolactin by Site-Directed Mutagenesis
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概要
- 論文の詳細を見る
To investigate the roles of the amino acids of rat prolactin (rPRL), the structure of which is presumed to consists of an atiparallel, four-α-helix bundle, mutations constructed by site-directed mutagenesis were assayed in terms of their receptor binding and Nb2 cell proliferation actibvities. Replacement of P64L (replaceing proline at position 64 with leucine) and K67E, which are located in the long loop region between helices 1 and 2, produced drastic decreases in the binding and proliferation activities. Mutations at D91 and E118 in the second and third helices, respectively, resulted in increased Nb2 cell proliferation activity with a slight increase in receptor binding activity. Mutations at L81 in the second helix and Y145 and W148 in the second loop between helics 3 and 4 produced no marked changes. Mutation at D158N in helix 4 markedly increased receptor binding activity with a slight loss of Nb2 cell proliferation activity, although two other mutations, D158H and D158R, produced a decrease of receptor binding activity without notable changes in Nb2 cell proliferation activity. These results demonstrate that P64 and K67 are crucial for PRL function while replacement of D91, E118 and D158 possibly leads to functional enhancement.
- 社団法人日本動物学会の論文
著者
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KATO Yukio
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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KATO Takako
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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TOMIZAWA Kyoko
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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Sato Takanobu
Lab. Of Molecular Biology And Gene Regulation Graduate School Of Agriculture
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Susa Takao
明治大学 農学部生命科学科
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Kato Y
Meiji Univ. Kanagawa Jpn
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Tomizawa K
Gunma Univ. Gunma Jpn
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Susa Takao
Division Of Life Science Graduate School Of Agriculture Meiji University
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Sato T
Inst. Of Reproduction And Endocrinology Meiji Univ.
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Kato Takako
Institute Of Reproduction And Endocrinology Meiji University
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Kato Y
Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci
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Tomizawa Kyoko
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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Kato Yukio
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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