Regulation of Porcine Pituitary Glycoprotein Hormone Alpha Subunit Gene with LIM-Homeobox Transcription Factor Lhx3

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概要

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• The aim of this study was to characterize the promoter activity of the porcine pituitary glycoprotein hormone common alpha gene (Cga) promoter (-1059/+12) and the role of LIM homeodomain transcription factor Lhx3. A transfection assay using Chinese hamster ovary (CHO) cells showed that the -1059/-101 region of the Cga promoter definitely responds to Lhx3 and that the -1059/-240 region exhibits a high basal transcriptional level in a pituitary-derived cell line, LβT2. A DNA binding and DNase I footprinting assay demonstrated that Lhx3 has seven binding sites in the -1059/+12 region of Cga, including a pituitary glycoprotein hormone basal element (PGBE) known as a LIM homeodomain factor-binding site. A transfection assay of the sequence of Lhx3-binding sites fused with minimal promoter vector confirmed their Lhx3-dependent stimulations in LβT2 cells. RT-PCR analysis of porcine pituitary ontogeny demonstrated that porcine Lhx3 showed striking changes of expression in both sexes during the fetal period but a stable high level of expression after birth. Thus, the porcine Cga promoter is regulated by Lhx3 through seven sites in the distal and proximal regions.

• 日本繁殖生物学会の論文
• 2009-08-01

著者

• Ishikawa Akio

  Division Of Life Science Graduate School Of Agriculture Meiji University

• Kato T

  Institute Of Reproduction And Endocrinology Meiji University

• KATO Yukio

  Division of Pharmaceutical Sciences, Graduate School of Natural Science & Technology

• SUSA Takao

  Division of Life Science, Graduate School of Agriculture, Meiji University

• KATO Takako

  Institute of Reproduction and Endocrinology, Meiji University

• NAKAYAMA Michie

  Division of Life Science, Graduate School of Agriculture, Meiji University

• KITAHARA Kousuke

  Division of Life Science, Graduate School of Agriculture, Meiji University

• Sato Takanobu

  Lab. Of Molecular Biology And Gene Regulation Graduate School Of Agriculture

• Susa Takao

  明治大学 農学部生命科学科

• Kato Yukio

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Kato Y

  Meiji Univ. Kanagawa Jpn

• Susa Takao

  Division Of Life Science Graduate School Of Agriculture Meiji University

• Kato Yukio

  Division Of Life Science Graduate School Of Agriculture Meiji University

• Kato T

  Waseda Univ. Tokyo Jpn

• Sato T

  Inst. Of Reproduction And Endocrinology Meiji Univ.

• Kato Takako

  Institute Of Reproduction And Endocrinology Meiji University

• Kato Yukio

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Kitahara Kousuke

  Division Of Life Science Graduate School Of Agriculture Meiji University

• Kato Takako

  Institute Of Plasma Physics Nagoya University

• Kato Y

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Nakayama Michie

  Division Of Life Science Graduate School Of Agriculture Meiji University

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