ウシ体細胞核移植卵由来胚盤胞の凍結保存:発育ステージが凍結融解後の生存性に及ぼす影響
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概要
- 論文の詳細を見る
The effect of developmental stage on the survival of bovine somatic cell nuclear-transferred blastocysts after freezing and thawing was evaluated. We also investigated how freezing affects nuclear-transferred (NT) embryos and in vitro fertilized (IVF) bovine embryos. Advanced-stage bovine NT blastocysts survived freezing better than early-stage NT blastocysts (86 vs 14%). The trend was similar with IVF embryos (87 vs 30%). At the stages tested, there was no significant difference in the survivability of NT and IVF embryos from advanced (86 vs 87%) or early-stage blastocysts (14 vs 30%). The average survival rate did not differ between NT and IVF bovine embryos (50 vs 51%). The higher survival rate of advanced-stage blastocysts compared to early-stage blastocysts in NT and IVF bovine embryos might be due to their higher cell number. In NT (128 ± 25 vs 53 ± 20) and IVF (128 ± 29 vs 75 ± 22) groups, advanced-stage blastocysts contained a significantly higher total cell number than early-stage blastocysts. There was no difference in total cell number between advanced-stage NT and IVF blastocysts (128 ± 25 vs 128 ± 29), however, early-stage NT and IVF blastocysts (53 ± 20 vs 75 ± 22) differed significantly.
- 日本繁殖生物学会の論文
- 2004-10-01
著者
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加藤 容子
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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角田 幸雄
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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Amarnath Dasari
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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Amarnath Dasari
近畿大学 農学部
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Amarnath Dasari
Laboratory Of Animal Reproduction College Of Agriculture Kinki University
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TSUNODA Yukio
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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KATO Yoko
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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Kamiya Yoshikazu
Department Of Hematology Meitetsu Hospital
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Kato Y
Meiji Univ. Kanagawa Jpn
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Kato Y
Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci
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Tsunoda Yukio
Lab. Of Animal Reproduction Coll. Of Agriculture Kinki Univ.
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Tsunoda Yukio
Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme
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Kato Yoko
Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme
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