マウスES細胞由来核移植卵の発生能に及ぼす核移植方法の影響

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概要

• 論文の詳細を見る

• Enucleated oocytes receiving mouse embryonic stem (ES) cells develop into fertile young. The developmental potential to young is low, however, and the rate of postnatal death is high. We examined the effect of various nuclear transfer procedures on the in vitro and in vivo developmental potential of nuclear-transferred oocytes. The potential of oocytes receiving ES cells at M phase to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection (67% vs. 30%). The developmental potential of oocytes receiving ES cells at the M phase is higher than that of oocytes receiving ES cells at the G1 phase (30-67% vs. 2-5%). Developmental ability to live young was low in all groups (0-4%). Different activation protocols affected the potential to develop into blastocysts to a different extent (27-62%), but did not affect the potential to develop into live young (0-3%). The present study demonstrated that the various conditions examined did not affect the potential of nuclear-transferred oocytes receiving ES cells to develop into live young or the incidence of postnatal death.

• 日本繁殖生物学会の論文
• 2004-04-01

著者

• 加藤 容子

  Laboratory of Animal Reproduction, College of Agriculture, Kinki University

• 薮内 晶子

  Laboratory of Animal Reproduction, College of Agriculture, Kinki University

• 安田 佳子

  First Department of Anatomy, School of Medicine, Kinki University

• KATO Yoko

  Laboratory of Animal Reproduction, College of Agriculture, Kinki University

• Tsunoda Y

  Laboratory Of Animal Reproduction College Of Agriculture Kinki University

• YABUUCHI Akiko

  Laboratory of Animal Reproduction, College of Agriculture, Kinki University

• YASUDA Yoshiko

  First Department of Anatomy, School of Medicine, Kinki University

• Kato Y

  Laboratory Of Animal Reproduction College Of Agriculture Kinki University

• Yasuda Y

  First Department Of Anatomy School Of Medicine Kinki University

• Yasuda Yoshiko

  First Department Of Anatomy Kinki University School Of Medicine

• Yabuuchi A

  Laboratory Of Animal Reproduction College Of Agriculture Kinki University

• Tsunoda Yukio

  Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme

• Kato Yoko

  Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme

関連論文

• ブタ単為発生卵と核移植卵の体外発生能に及ぼす共培養の影響
• ウシ体細胞核移植卵由来胚盤胞の凍結保存:発育ステージが凍結融解後の生存性に及ぼす影響
• マウスES細胞由来核移植卵の発生能に及ぼす集合ならびに再核置換の影響
• マウスES細胞由来核移植卵の発生能に及ぼす核移植方法の影響
• Gene Expression in Individual Bovine Somatic Cell Cloned Embryos at the 8-cell and Blastocyst Stages of Preimplantation Development
• Effect of the Timing of First Cleavage on In vitro Developmental Potential of Nuclear-Transferred Bovine Oocytes Receiving Cumulus and Fibroblast Cells
• Aging of Recipient Oocytes Reduces the Development of Cloned Embryos Receiving Cumulus Cells
• ブタ単為発生卵ならびに胎子繊維芽細胞由来核移植卵の体外発生能に及ぼす酸素気相の影響
• The Recent Progress on Nuclear Transfer in Mammals
• Nuclear Transplantation of Mouse Inner Cell Mass and Trophectoderm Cells into Enucleated Two-Cell Embryos
• Effects of the Age of Donor Embryos on the Developmental Ability of Bovine Nuclear Transferred Eggs In Vitro
• EFFECTS OF THE NEONATAL VITAMIN A EXPOSURE ON BEHAVIORS OF ADULT RATS
• ウシ体細胞核移植卵由来胚盤胞の凍結保存:発育ステージが凍結融解後の生存性に及ぼす影響
• Effects of Several Factors on the Monozygotic Twin Production in the Mouse
• cDNAマイクロアレイによるウシ栄養膜細胞株(BT-1)の遺伝子発現プロファイル
• Development of Enucleated Mouse Oocytes Receiving PDGF or FGF Treated Fetal Male Germ Cells after Activation with Electrical Stimulation
• Nuclear Transplantation of Mouse Inner Cell Mass and Trophectoderm Cells into Enucleated Two-Cell Embryos
• Erythropoietin/Erythropoietin-receptor system as an angiogenic factor in chemically induced murine hepatic tumors
• Effects of the Age of Donor Embryos on the Developmental Ability of Bovine Nuclear Transferred Eggs In Vitro
• マウスES細胞由来核移植卵の発生能に及ぼす核移植方法の影響
• Pluripotency of mouse embryonic cells on germline at 3.5-8.5 and 11.5 days post-coitum after aggregation with precompacted embryos Yoko
• Gene Expression Profiles of Bovine Trophoblastic Cell Line (BT-1) Analyzed by a Custom cDNA Microarray

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