ブタ単為発生卵と核移植卵の体外発生能に及ぼす共培養の影響

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記事区分：原著[Author abstract]Effect of co-culture with somatic cells on the in vitro developmental potential of parthenogenetic and nuclear transferred embryos in pig was examined. Single blastomere from 4- to 32- cell-stage embryos, which were collected from artificially inseminated female gilts at day three to four, was fused with enucleated oocytes matured in vitro. Fused oocytes were cultured with somatic cells for 8 days in vitro, or some nuclear transferred embryos were transferred to synchronized recipient gilts after overnight culture in vitro. Results demonstrated that nuclear transferred embryos cleaved at a significantly higher rate under co-culture system with pig cumulus (41 vs 63%) and bovine oviductal cells (41 vs 59%). Later development beyond 4-cell stage was, however, limited and similar (5 vs 7 and 10%), and no blastocyst was obtained. While, parthenogenetic oocytes developed to blastocysts with pig cumulus cells (7%), bovine oviductal cells (4%) and even without co-culture (10%). When nuclear transferred embryos cultured overnight were transferred to 8recipient gilts, estrus of gilys returned on day 20 to 58 days and no pregnancy was observed.[著者抄録]本研究では,ブタ単為発生卵と初期はい割球をドナー核とした核移植卵の体外発生能に及ぼす共培養細胞の影響について検討した。共培養細胞には,ブタ卵管細胞,ブタ卵丘細胞,ウシ卵管細胞を用いた。その結果,共培養細胞にブタ卵丘細胞あるいはウシ卵管細胞を用いると,核移植卵の分割率を有意に向上させることができた。しかしながら,はい盤胞への発生率を向上させるには至らず,今後,更に検討を要することが明らかとなった。
近畿大学の論文
2004-03-31

ブタ単為発生卵と核移植卵の体外発生能に及ぼす共培養の影響

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