Gene Expression in Individual Bovine Somatic Cell Cloned Embryos at the 8-cell and Blastocyst Stages of Preimplantation Development
スポンサーリンク
概要
- 論文の詳細を見る
Aberrant gene expression in somatic cell nuclear-transferred (NT) embryos due to abnormal epigenetic modifications of the donor nucleus likely accounts for much of the observed diminished viability and developmental abnormalities. We compared the expression of 13 developmentally important genes in individual 8-cell and blastocyst stage NT embryos produced from adults female cumulus cells and adult male skin fibroblast cells with low and high incidences of neonatal abnormalities [1, 2]. In vitro-fertilized (IVF) embryos were used as control embryos. Among the genes tested, the relative abundance of Glut-1, IGF-1R, E-cad, and Cx43 transcripts varied significantly between the two types of NT embryos at the 8-cell stage. The relative abundance of manganese super oxide dismutase (MnSOD) and Stat3 transcripts was significantly higher in IVF embryos compared with both types of NT embryos. At the blastocyst stage, there was a significant difference in the relative expression of only one gene, Bcl-2, between the two types of NT embryos. Although the level of Glut-1 expression did not vary between the two types of NT blastocysts, its expression in both types of NT blastocysts was significantly lower than that in IVF blastocysts. The MnSOD expression level tended to be higher in NT blastocysts. The gene expression profile for any single gene, however, was highly variable among individual embryos and was independent of embryo morphology. The present study demonstrated that the expression profiles of the 13 genes examined in Day 9 NT blastocysts produced from two different types of donor cells with different incidences of neonatal abnormalities are largely indistinguishable.
- 日本繁殖生物学会の論文
- 2007-12-01
著者
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Amarnath Dasari
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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Amarnath Dasari
近畿大学 農学部
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Amarnath Dasari
Laboratory Of Animal Reproduction College Of Agriculture Kinki University
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TSUNODA Yukio
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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LI Xiangping
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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KATO Yoko
Laboratory of Animal Reproduction, College of Agriculture, Kinki University
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Kato Y
Meiji Univ. Kanagawa Jpn
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Li Xiangping
Laboratory Of Animal Reproduction College Of Agriculture Kinki University
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Kato Y
Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci
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Tsunoda Yukio
Lab. Of Animal Reproduction Coll. Of Agriculture Kinki Univ.
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Tsunoda Yukio
Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme
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Kato Yoko
Laboratory Of Animal Reproduction College Of Agriculture And Research Institute For Animal Developme
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