Analysis of Genes Expressed During Porcine Fetal Pituitary Development by Suppressive Subtraction Hybridization

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概要

• 論文の詳細を見る

• Pituitary organogenesis is modulated by temporal and special expression of various genes at the fetal stage. Suppressive subtraction cloning was employed to search for genes expressed during porcine fetal pituitary development. The variations in the genes expressed in the pituitaries on fetal days 40 and 110, respectively, were analyzed by subtraction with the inverted combination. Genes encoding growth arrest specific-2, coated vesicle membrane protein, steroid membrane binding protein, putative protease cancer tumor suppressor, secretogranin, calumenin and others exhibited elevated expression during pituitary development, in addition to genes encoding GH, prolactin and glycoprotein hormone common α subunit. Concomitantly, we also found a gene that encodes a porcine ortholog of c-myc transcription factor, and determined its entire nucleic acid sequence. On the other hand, histone 3.3B, GTP-binding α-stimulatory subunit, stathmin and so on were found as gene expression decreased. Thus, the expression levels of many genes change during pituitary development.

• 日本繁殖生物学会の論文
• 2007-10-01

著者

• Kato T

  Institute Of Reproduction And Endocrinology Meiji University

• KATO Takako

  Institute of Reproduction and Endocrinology, Meiji University

• TAKAHASHI Jun

  Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture, Meiji Universit

• SUSA Takao

  Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture, Meiji Universit

• KATO Yukio

  Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture, Meiji Universit

• SATO Takanobu

  Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture

• ASANO Hiroko

  Department of Life Science, School of Agriculture, Meiji University

• ELSAESSER Folkmar

  Institute for Animal Science and Animal Behavior

• Sato Takanobu

  Lab. Of Molecular Biology And Gene Regulation Graduate School Of Agriculture

• Susa Takao

  明治大学 農学部生命科学科

• Kato Yukio

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Kato Y

  Meiji Univ. Kanagawa Jpn

• Susa Takao

  Division Of Life Science Graduate School Of Agriculture Meiji University

• Asano Hiroko

  Department Of Life Science School Of Agriculture Meiji University

• Kato T

  Waseda Univ. Tokyo Jpn

• Sato T

  Inst. Of Reproduction And Endocrinology Meiji Univ.

• Kato Takako

  Institute Of Reproduction And Endocrinology Meiji University

• Kato Yukio

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Kato Takako

  Institute Of Plasma Physics Nagoya University

• Kato Y

  Department Of Dental And Medical Biochemistry Hiroshima University Graduate School Of Biomedical Sci

• Kato Yukio

  Laboratory Of Molecular Biology And Gene Regulation Department Of Life Science Faculty Of Agricultur

• Takahashi Jun

  Laboratory Of Molecular Biology And Gene Regulation Graduate School Of Agriculture Meiji University

• SUSA Takao

  Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan

• SATO Takanobu

  Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University

• SUSA Takao

  Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University

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