マウス乳頭・乳腺組織におけるプロラクチン遺伝子発現の解析
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概要
- 論文の詳細を見る
In this study, we attempted to examine the presence of prolactin (PRL) messenger ribonucleic acid (mRNA) and protein in the mouse nipple and mammary gland in pregnancy and lactation. PRL-like substances were found by immunohistochemistry using an antibody against the mouse PRL (mPRL) in the sebaceous gland cells of the nipple during late pregnancy and lactation, and the cistern of alveoli in mammary glands during lactation. Western blot analysis of proteins extracted from the nipple and the mammary gland showed immunoreactive bands corresponding to molecular weights of approximate 16 kDa and 32 kDa, respectively. The expression of mRNA for mPRL in the nipple and mammary gland during late pregnancy and lactation was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR), Southern blotting, and nucleotide sequence analyses. These results suggest that mPRL mRNA and its translation product are synthesized in the mouse nipple.
- 日本繁殖生物学会の論文
著者
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KATO Yukio
Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture, Meiji Universit
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冨田 陽子
明治大学農学部生命科学科生体機構学研究室
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小泉 稔
明治大学農学部生命科学科生体機構学研究室
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小泉 稔
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
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堀口 幸太郎
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
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冨田 陽子
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
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HARIGAYA Toshio
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
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Horiguchi Kotaro
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
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KOIZUMI Minoru
Laboratory of functional Anatomy, Faculty of Agriculture, Meiji University
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TOMITA Yoko
Laboratory of functional Anatomy, Faculty of Agriculture, Meiji University
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Harigaya Toshio
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
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堀口 幸太郎
明治大学 農学部生命科学科生体機構学研究室
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Kato Yukio
Laboratory Of Molecular Biology And Gene Regulation Department Of Life Science Faculty Of Agricultur
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Horiguchi Kotaro
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
関連論文
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- マウス乳頭・乳腺組織におけるプロラクチン遺伝子発現の解析
- マウス乳頭・乳腺組織におけるプロラクチン遺伝子発現の解析
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