Estrogen Regulates the Serum Level of Phosphorylated Prolactin in Mice
スポンサーリンク
概要
- 論文の詳細を見る
Phosphorylated prolactin (PPRL) is considered to be the most quantitatively important post-translationally modified form of prolactin (PRL) in rodents. We recently detected two different types of PPRL in the mouse pituitary gland; one was phosphorylated at serine and the other was phosphorylated at serine/threonine. Furthermore, we showed that there are obvious differences in the ratios between PPRLs and non-phosphorylated PRL in the pituitary gland based on age and sex and that estrogen influences PRL phosphorylation at serine in female mice. In the present study, we examined whether estradiol (E2) increases serine PPRL in the male pituitary gland in the same manner as in the female pituitary gland and examined whether PPRL is released into serum. We first determined the relative amounts of intrapituitary PPRLs in male mice under different pharmacological conditions that increased PRL secretion. The results indicated that treatment with E2 increases serine PPRL. We then performed two-dimensional electrophoresis and immunoblotting analysis after immunoprecipitation with anti-mouse PRL antibody using male and female sera under different pharmacological conditions that increased PRL secretion. The results of this experiment indicated that there were PRLs phosphorylated at serine and serine/threonine in the female serum but not in the male serum. The levels of PPRLs in sera were greatly increased with the E2 treatment for both male and female sera. Furthermore, we examined the effect of E2 on PPRL synthesis in cultured male pituitary glands. In this experiment, we observed increased serine PPRL synthesis and stronger immunohistochemical staining of PRL cells with E2 treatment. These findings suggested that serine PPRL synthesis and secretion were influenced by estrogen.
- 2007-08-01
著者
-
YOSHIDA Makoto
Laboratory of Aquatic Biology and Environmental Science, Graduate School of Agricultural and Life Sc
-
HARIGAYA Toshio
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
-
Kosugi Tatsuya
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Horiguchi Kotaro
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
-
Ishida Michiyo
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
-
Harigaya Toshio
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Harigaya Toshio
Faculty Of Agriculture Meiji University
-
Harigaya T
Meiji Univ. Kawasaki Jpn
-
Hasegawa T
Japan Racing Horse Assoc. Utsunomiya‐shi Jpn
-
Hasegawa T
Equine Research Institute
-
Naito J
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Hosoyama Tohru
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
-
Ishida M
Meiji Univ. Kawasaki Jpn
-
Harigaya T
Department Of Anatomy Faculty Of Medicine Kyoto University
-
FUKUTA Shinya
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
-
NAITO Jun-ichiro
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji Univers
-
Ishida Michiyo
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Fukuta Shinya
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Horiguchi Kotaro
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Yoshida Makoto
Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University
-
Hasegawa Telhisa
Equine Res. Inst. Japan Racing Assoc.
-
HORIGUCHI Kotaro
Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo 192-8508, Japan
関連論文
- Construction and Validation of Parentage Testing for Thoroughbred Horses by 53 Single Nucleotide Polymorphisms
- The first finding of toxic dinoflagellate Alexandrium minutum in Vietnam
- マウス乳頭・乳腺組織におけるプロラクチン遺伝子発現の解析
- ウマ(サラブレッド)マイオスタチンcDNAのクローニングと血清中マイオスタチン前駆タンパク質の検出
- Identification of Prolactin Synthesis at Early Differentiation Stage of T Cells in the Thymus
- Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes
- Isolation of Satellite Cells from Equine Skeletal Muscle
- ウマ(サラブレッド種)各組織におけるc-ski遺伝子の発現について
- LOCALIZATION OF PROLACTIN - LIKE SUBSTANCE PRODUCING CELLS IN MOUSE NIPPLE
- Prolactin Binding Sites in Normal Uterus and the Uterus with Adenomyosis in Mice
- Clinical Application of Chromogranin A as an Indicator of Stress : Changes in Autonomic Nervous Activity During Copulatory Behavior in Stallions
- Demographic Analysis of Breeding Structure in Japanese Thoroughbred Population
- Renal Nerve Stimulation Induces α_2-Adrenoceptor-Mediated Antinatriuresis under Inhibition of Prostaglandin Synthesis in Anesthetized Dogs
- The mRNA Expression of Neurocan, a Brain-Specific Chondroitin Sulfate Proteoglycan, in Neoplastic Mammary Glands in Mice
- Mouse TGF_α Gene Expression in Normal and Neoplastic Mammary Glands and Uteri of Four Strains of Mice with Different Potentials for Mammary Gland Growth and Uterine Adenomyosis
- Different Gene Expression of Mouse Transforming Growth Factor α between Pregnant Mammary Glands and Mammary Tumors in C3H/He Mice
- A Thoroughbred Showing Development Disorder in the Pudendal Cleavage due to Chromosomal Mosaicism
- LPSとサイトカインで刺激されたウマ培養細胞におけるNO産生
- Changes in Serum Concentration of Uric Acid and Allantoin due to Exhaustive Treadmill Exercise
- Molecular Cloning, Nucleotide Sequence and Tissue Distribution of Equine Testicular 3β-Hydroxysteroid Dehydrogenase/Δ5-Δ4 Isomerase Messenger Ribonucleid Acid
- Long Term Adrenal Insufficiency Induces Skeletal Muscle Atrophy and Increases the Serum Levels of Active Form Myostatin in Rat Serum(Physiology)
- Participation of Angiotensin II in Pressor Response and Norepinephrine Release to Spinal Nerve Stimulation in Pithed Rats
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- Role of Serum Myostatin during the Lactation Period
- Estrogen Regulates the Serum Level of Phosphorylated Prolactin in Mice
- Linear SRY Transcript in Equine Testis
- Analysis of Prolactin Gene Expression and Cleaved Prolactin Variants in the Mouse Testis and Spermatozoa
- The Effect of Estrogen on Phosphorylation of Prolactin in the Mouse Pituitary Gland
- Expression Profiling of Mouse Placental Lactogen II and Its Correlative Genes Using a cDNA Microarray Analysis in the Developmental Mouse Placenta
- 妊娠後半期マウス胎盤におけるカテプシン遺伝子発現
- cDNA MICROARRAY ANALYSIS IN THE DEVELOPMENTAL MOUSE PLACENTA
- IDENTIFICATION OF PROLACTIN PRODUCING CELLS IN THE MOUSE SPLEEN
- SUBTARCTION ANALYSIS OF MOUSE PLACENTAL GENE EXPRESSION IN MID PREGNANCY
- SUBTRACTION ANALYSIS AND CHARACTERIZATION OF SPECIFIC GENE EXPRESSION IN MOUSE PLACENTA BETWEEN MID AND LATE PREGNANCY
- Prolactin gene expression in mouse pancreatic islets
- Expression of Prolactin Gene in Mouse Placenta During Late Pregnancy : Detection of mRNA and Its Translation Product
- ECTOPIC PROLACTIN GENE EXPRESSION IN MICE
- Type VII and XVII Collagen mRNA Expressions in Regenerated Epidermal Laminae in Chronic Equine Laminitis
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Gene Dropping Analysis of Ancestral Contributions and Allele Survival in Japanese Thoroughbred Population
- Analysis of Prolactin Gene Expression and Cleaved Prolactin Variants in the Mouse Testis and Spermatozoa
- Satellite Cell Differentiation in Goat Skeletal Muscle Single Fiber Culture
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- Genetic Characterization of Endangered Kiso Horse Using 31 Microsatellite DNAs
- IMMUNOHISTOCHEMICAL STUDY OF POSTNATAL DEVELOPMENT OF PROLACTIN-PRODUCING CELLS IN C57BL MICE
- Specific Binding of Prolactin to the Mammary Gland and Lactose Synthesis in Pregnant Rats After Removal of Ovaries or Ovaries and Adrenals
- Population Statistics and Biological Traits of Endangered Kiso Horse
- Establishment of bipotent progenitor cell clone from rat skeletal muscle
- Prolactin Gene Expression in Mouse Mammary Glands during Late Pregnancy and Lactation
- Molecular Cloning of Rat and Porcine Retina-derived POU Domain Factor 1 (POU6F2) from a Pituitary cDNA Library