Linear SRY Transcript in Equine Testis

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概要

• 論文の詳細を見る

• Employing a combination of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques, the complete coding sequence of cDNA for the equine SRY gene was determined. We also attempted to make clear whether the equine SRY gene transcript is expressed in the adult testis, and whether the type of transcript is expressed as linear or circular RNA. As a result, in total a 1420 bp cDNA sequence was determined. Accomplishment of 3 RACE infers that equine SRY gene was expressed as a linear RNA transcript in testicular tissue just after puberty, in contrast to the situation in mice.

著者

• Harigaya Toshio

  Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University

• Ishida Nobushige

  Laboratory Of Molecular And Cellular Biology Equine Research Institute

• Hasegawa Telhisa

  Laboratory Of Molecular And Cellular Biology Equine Research Institute Japan Racing Association

• Mukoyama Harutaka

  Laboratory Of Animal Physiology Tokyo University Of Agriculture

• Ishida Michiyo

  Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University

• SATO Fumio

  Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association

• ISHIDA Michiyo

  Laboratory of Functional Anatomy, Faculty of Agriculture, Meiji University, Kawasaki 214-0033, Japan

• ISHIDA Nobushige

  Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya 320-0856, Japan

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