In Vitro Development of Equine Oocytes from Preserved Ovaries after Intracytoplasmic Sperm Injection
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概要
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In this study, we evaluated the meiotic competence of equine oocytes from ovaries preserved for one day. We also investigated fertilization, cleavage rate, developmental competence and freezability of equine embryos after intracytoplasmic sperm injection (ICSI). After collection from ovaries, the oocytes were classified into two groups comprised of those having compact cumulus layers (Cp) or those having expanded cumulus layers (Ex). Oocytes with a first polar body were subjected to fertilization by ICSI using frozen-thawed stallion spermatozoa and were then cultured in CR1aa medium. The rates of metaphase II-stage oocytes, normal fertilization and cleavage were not significantly different between the two oocyte categories (38.5, 70.0 and 48.7% for CP and 43.5, 60.0 and 58.8% for Ex, respectively). However, the blastocyst development rate of Ex was significantly (P<0.05) higher than that of Cp (25.5% vs. 7.7%). Three Cp-derived and 12 Ex-derived early blastocysts were cryopreserved using the slow cooling protocol, and all of them developed to hatching blastocysts after thawing. These results suggest that equine oocytes fertilized by ICSI can develop to the preimplantation stage in culture conditions similar to those used in the bovine. Furthermore, the Ex oocytes had higher developmental competence than the Cp oocytes, and the in vitro-produced blastocysts had high viability after freezing and thawing.
- 日本繁殖生物学会の論文
- 2007-08-01
著者
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SATO Fumio
Laboratory of Racing Chemistry
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Akagi Satoshi
Department of Cardiovascular Medicine, Okayama University
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Akagi Satoshi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Akagi Satoshi
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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HASEGAWA Telhisa
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Horse Associat
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Adachi Noritaka
Ibaraki Prefectural Livestock Research Center
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Takahashi Seiya
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Takahashi Seiya
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Hasegawa Telhisa
Laboratory Of Molecular And Cellular Biology Equine Research Institute Japan Racing Association
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Matsukawa Kazutsugu
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Matsukawa Kazutsugu
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Sato Fumio
Hidaka Training And Res. Centre Japan Racing Assoc.
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SATO Fumio
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association
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