Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
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概要
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The expression of inhibin α-subunit mRNA was examined in the corpus luteum (CL) of pregnant mares. Corpora lutea were obtained from pregnant mares at 50 h, 94 h after ovulation and on 30, 60, and 90 days of pregnancy. Northern blot analysis, using a cDNA specific to equine inhibin α-subunit as a probe revealed that the expression level of its transcript continued to decline until 30 days after ovulation. However, on day 60 of pregnancy, the level increased similar to that at 94 h after ovulation. On day 90 of pregnancy, the level declined again. The expression of inhibin α-subunit mRNA in the estrous cycle CL (7 days after ovulation) was also examined but the level was moderately low. Considering that serum concentration of equine chorionic gonadotropin (eCG) peaks at around day 60 of pregnancy, and the expression level of inhibin α-subunit in the CL of the pregnant mare also shows a peak on day 60 of pregnancy, it was suggested that, during pregnancy in equidae, the expression of inhibin α-subunit mRNA in the CL is stimulated by eCG.
- 日本繁殖生物学会の論文
著者
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NISHIHARA Masugi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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YAMANOUCHI Keitaro
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
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TAKAHASHI Michio
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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HASEGAWA Telhisa
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Horse Associat
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IKEDA Akihiro
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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MATSUYAMA SHIGEMI
Department of Veterinary Physiology,Veterinary Medical Science,The University of Tokyo,
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Miyazawa Kiyoshi
Department Of Veterinary Reproduction
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Yamanouchi K
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
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Ikeda Akihiro
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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SUGAWARA Yukihiro
Laboratory of Livestock Biotechnology, Institute of Animal Resource Sciences, Graduate School of Agr
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SAWASAKI Toru
Laboratory of Livestock Biotechnology, Institute of Animal Resource Sciences, Graduate School of Agr
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