Possible Involvement of Amiloride-Sensitive Na<SUP>+</SUP>/H<SUP>+</SUP> Exchanger and/or Na<SUP>+</SUP> Transport Systems in Hatching and the Trophoblast Spreading in Mouse Blastocysts Cultured <I>in vitro</I>
スポンサーリンク
概要
- 論文の詳細を見る
In mouse blastocysts cultured <I>in vitro</I>, trophoblast cells undergo extensive spreading which represents the invasion stage of implantation <I>in vivo</I>. The trophoblast spreading is accompanied by striking increase of cellular volume and transformation of the cells into the giant trophoblast cells. Since, Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger system (or Na<SUP>+</SUP>/H<SUP>+</SUP> antiporter) on the plasma membrane of vertebrate cells plays a crucial role in regulating cellular volume in mammalian cells, the carrier mediated Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger system might be involved in the process of trophoblast spreading. To obtain clues to approach the problem, we examined effects of amiloride which is a potent inhibitor of Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger and other amiloride sensitive Na<SUP>+</SUP> channels, upon mouse blastocysts cultured <I>in vitro</I>. Amiloride inhibited, in dose-dependent manner, hatching as well as the trophoblast spreading in mouse blastocysts cultured <I>in vitro</I>. Our findings strongly indicated that the amiloride sensitive Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger system might be operating, in particular, during the initial phase of trophoblast spreading: the system appeared less active in the fully spread cells. To corroborate the view, experiments with more specific inhibitors of Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger are currently in progress in our laboratory. In addition, while amiloride and its derivatives have been used as diuretic drugs, little has been known concerning their effects upon the peri-implantation embryos. Our data clearly indicates their potentially adverse effects upon the conceptuses during early phase of gestation.
- 日本繁殖生物学会の論文
著者
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Tojo H
Univ. Tokyo Tokyo Jpn
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Tojo Hideaki
東京大学 農学生命科学研究科応用遺伝学
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Tanaka S
Univ. Tokyo Tokyo Jpn
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舘 鄰
Life Science Institute Mitsubishi-kagaku Co. Ltd
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Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
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Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Tojo Hideaki
Laboratory Of Animal Reproduction
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Tachi C
Azabu Univ. Sagamihara Jpn
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Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
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Tanaka Satomi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
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Tachi S
Department Of Anatomy Tokyo Women's Medical College
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Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
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TACHI Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agriculture, University of Tokyo
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TANAKA Satomi
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agriculture, University of Tokyo
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