乳清タンパク質(WAP)を全身性に過剰発現するトランスジェニックマウスにおける乳腺の発達異常とβ-カゼインの早期発見
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概要
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It has been suggested that whey acidic protein (WAP) may function as a protease inhibitor. However, the actual function of WAP remains obscure. We investigated the histological development of the mammary glands of transgenic mice ubiquitously expressing WAP and CAG/WAP transgene. Ubiquitous expression of WAP induced aberrant development of the lobular alveoli of the mammary glands: mammary alveoli that were either aberrantly large or small in size increased in number in the developing mammary glands of these transgenic females during pregnancy and lactation. The expression of β-casein was precociously induced in the mammary glands of the transgenic females during early pregnancy and accompanying this was a histological observation that abnormally developed lobular alveoli filled with milk proteins appeared in the mammary glands of transgenic females during early pregnancy. However, during lactation, the development of mammary glands was impaired in transgenic females. To investigate the possible paracrine action of WAP associated with mammary gland aberration, we transplanted the mammary tissue of CAG/EGFP transgenic females into the fat pad of virgin CAG/WAP transgenic females and initiated pregnancy by mating. The development of mammary tissue transplanted to the recipient was histologically examined on day 3 of lactation. The results revealed that the development of grafted mammary tissues was impaired in a manner similar to that of the mammary glands of CAG/WAP transgenic females, indicating that the inhibitory effect of WAP acts via a paracrine mechanism. In vitro experiments using HC11 cells with forced expression of exogenous WAP demonstrated the inhibitory function of WAP on proliferation of mammary epithelial cells.
著者
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KANO Kiyoshi
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Toky
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岩森 督子
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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大澤 恵美
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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生見 尚子
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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SUDO Katsuko
Animal Research Center, Tokyo Medical University
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NAITO Kunihiko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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岩森 督子
東京大学・大学院農学生命科学研究科・応用遺伝学研究室
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生見 尚子
東京大学・大学院農学生命科学研究科・応用遺伝学研究室
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Sudo Katsuko
Animal Res. Cent. Tokyo Med. Univ.
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Tojo Hideaki
Laboratory Of Animal Reproduction
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岩森 督子
麻布大学獣医学部発生工学研究室
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Naito Kunihiko
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Science University Of Tokyo
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NUKUMI Naoko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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IWAMORI Tokuko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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OOSAWA Megumi
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Kano Kiyoshi
Laboratory Of Applied Genetics Department Of Animal Resource Science Graduate School Of Agriculture
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Naito Kunihiko
Laboratory Of Applied Genetics Department Of Animal Resource Sciences Graduate School Of Agricultura
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