A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones
スポンサーリンク
概要
- 論文の詳細を見る
The limitations of studies of clarification of response elements of whey acidic protein (WAP) gene to hormones using mammary cell lines has been shown. We studied the response of the upstream region (2.6 kb) of WAP to various steroid hormones using gonadectomized mWAP/hGH transgenic mice. Ovariectomy or castration for transgenic mice was performed at 10 days or 30 days post partum. Various steroid hormones were administered daily for 10 days to the gonadectomized transgenic mice after they reached 2 months of age. Prior to the hormonal administration and 24 hr after the final administration, blood was collected and the hGH levels in the plasma was measured by RIA. Daily doses of estradiol-17β were significantly more effective at increasing hGH levels in transgenic females ovariectomized at 10 days post partum than progesterone of an equal dose. A combined dose of progesterone and of estradiol-17β significantly amplified the increase of hGH levels accompanied by the great development of mammary glands, compared to a dose of progesterone alone. Corticosterone induced only a slight increase of hGH, while testosterone had no effect. The doses of gonadal steroid hormones did not induce an increase in hGH levels and development of mammary glands in the castrated transgenic males. The results showed that the response of 5 region of WAP requires at least some extended development of the mammary gland and that the 2.6 kb upstream region of the exogenous WAP gene contained the element responsive to ovarian hormones.
- 社団法人 日本実験動物学会の論文
著者
-
TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
-
Tojo H
Univ. Tokyo Tokyo Jpn
-
Tojo Hideaki
東京大学 農学生命科学研究科応用遺伝学
-
YAMANOUCHI Keitaro
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
-
Yamanouchi K
Waseda Univ. Saitama
-
舘 鄰
Life Science Institute Mitsubishi-kagaku Co. Ltd
-
Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
-
Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Tojo Hideaki
Laboratory Of Animal Reproduction
-
Tachi C
Azabu Univ. Sagamihara Jpn
-
舘 鄰
東京大学大学院農学生命科学研究科応用動物科学専攻応用遺伝学研究室
-
Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
-
INUZUKA Hiroyuki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
-
Yamanouchi K
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
-
Yamanouchi Keitaro
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
-
舘 鄰
麻布大学獣医学部動物応用科学科
-
Yamanouchi Keitaro
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Yokouchi Kou
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
-
Inuzuka Hiroyuki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
-
Tojo Hideaki
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of Tokyo
関連論文
- Involvement of 20α-Hydroxysteroid Dehydrogenase in the Maintenance of Pregnancy in Mice
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- 乳清タンパク質(WAP)を全身性に過剰発現するトランスジェニックマウスにおける乳腺の発達異常とβ-カゼインの早期発見
- 哺乳類の毛皮標本からのDNA抽出ならびに機能遺伝子の回収に関する研究
- ウマ胎子生殖腺におけるインヒビンαサブユニットmRNAの発現とその局在
- Matrix metalloproteinases regulate mesonephric cell migration in developing XY gonads which correlates with the inhibition of tissue inhibitor of metalloproteinase-3 by sry
- ブタ卵に移植した卵丘細胞核へのサイクリンBとMAPキナーゼの局在
- 哺乳類卵子解析のための小規模ウエスタンブロッティング系「マイクロ・ウエスタンブロッティング」の確立
- ブタ卵成熟におけるMPF活性化に対する卵核胞の必要性の検討
- 哺乳類の毛皮標本からのDNA抽出ならびに機能遺伝子の回収に関する研究
- ウマフォリスタチンcDNAのクローニングと雌ウマ生殖器官における遺伝子発見
- ヤギ胎盤における20α-水酸化ステロイド脱水素酵素の発現とその役割
- リポソーム・ペプチド・DNA複合体は精子ベクター法による遺伝子導入効率を向上する
- 東京大学農学生命科学研究科に保存されているニホンオオカミ (Canis lupus hodophilax Temminck) 剥製標本から回収したアメロゲニン遺伝子(AMELX)エクソンDNAのクローニングとヌクレオチド配列の決定について
- ニホンカモシカ (Capricornis crispus) 精巣上体精子の液体窒素中での凍結保存
- ウマ(サラブレッド)マイオスタチンcDNAのクローニングと血清中マイオスタチン前駆タンパク質の検出
- 乳清酸性タンパク質(WAP)はマウスおよびヒトの乳癌細胞(MMT,MCF-7)の増殖を抑制する
- マウス乳腺上皮細胞(EpH4/K6)の増殖能に及ぼす乳清酸性タンパク質の抑制作用
- Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba Goats (Capra hircus var Shiba)
- シバヤギ胎盤から樹立したクローン化培養細胞株CPF-1におけるテロメア長短縮の解析
- シバヤギ胎盤から樹立したクローン化培養細胞株CPF-1におけるテロメア長短縮の解析
- 新生ラット視床下部における性ステロイド依存性遺伝子の発現解析
- 性腺刺激ホルモン放出ホルモンニューロンに緑色蛍光タンパク質を発現するトランスジェニックラットの作出
- ウマ(サラブレッド)マイオスタチンcDNAのクローニングと血清中マイオスタチン前駆タンパク質の検出
- マウス(Mus Musculus)血液漿膜胎盤およびシバヤギ(Capra hircus var Shiba)上皮漿膜/結合組織漿膜胎盤におけるホメオボックス遺伝子発現の解析
- プロジェステロンの前処置はエストロジェンにより誘導されるラット子宮上皮細胞でのc-ski mRNAの発現ならびに子宮上皮細胞の増殖を抑制する
- ブタ卵の減数分裂再開にはMAPキナーゼの活性が必要である
- Novel Development of Mammary Glands in the Nursing Transgenic Mouse Ubiquitously Expressing WAP Gene
- Analysis of Gender Difference of Cardiac Risk Biomarkers Using hGH-Transgenic Mice
- Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes
- Expression of Membrane-Type 1 Matrix Metalloproteinase (MT1-MMP) mRNA in Trophoblast and Endometrial Epithelial Cell Populations of the Synepitheliochorial Placenta of Goats (Capra hircus)
- Possible role for the c-ski gene in the proliferation of myogenic cells in regenerating skeletal muscles of rats
- A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones
- In vitro Studies on PGC or PGC-Like Cells in Cultured Yolk Sac Cells and Embryonic Stem Cells of the Mouse
- Isolation of Satellite Cells from Equine Skeletal Muscle
- Skeletal Muscles of Transgenic Mice Expressing Human snoN, a Homologue of c-ski
- ウマ(サラブレッド種)各組織におけるc-ski遺伝子の発現について
- Cell-Cell Contract Down-Regulates Expression of Membrane Type Metalloproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line
- Transgenic Mice Overexpressing Foreign Whey Acidic Protein (WAP) Genes Driven by Metallothionein Promoter
- ウマの卵胞発育過程におけるインヒビン/アクチビンサブユニット,アロマターゼおよび3β-ヒデロキシステロイド脱水素酵素の発現
- 妊娠中のマウス子宮と胎盤におけるβig-h3 mRNAの動態 : トロフォブラスト侵襲の制御因子としての可能性について
- Sex Difference and Postnatal Change of Maternal Behavioral Patterns in Juvenile Male and Female Rats(SHORT COMMUNICATION,Reproductive Biology)
- INCIDENCE OF RETRIEVING BEHAVIOR IN JUVENILE FEMALE AND MALE RATS : INFLUENCE OF NEONATAL ANDROGEN(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Functions of the N-Glycans of Rat Leukemia Inhibitory Factor Expressed in Chinese Hamster Ovary Cells
- Inhibitory Effect of Progesterone on Sexual Receptivity in Female Rats : A Temporal Relationship to Estrogen Administration
- マウス卵管を利用した牛IVF胚の体内培養
- Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11
- Expression of Matrix Metralloproteinase-11 (Stromelysin-3) and TIMP-1 Genes in the Placenta and the Uterus during Estrous Cycles and Gestation in the Mouse
- Normal and Neoplastic Growth of Mammary Glands and Circulating Levels of Prolactin and Growth Hormone in Mouse Whey Acidic Protein Promoter/Human Growth Hormone (mWAP/hGH) Transgenic Mice
- Bacteriostatic Activity of Whey Acidic Protein (WAP)
- Expression of Ski in the Granulosa Cells of Atretic Follicles in the Rat Ovary
- Long Term Adrenal Insufficiency Induces Skeletal Muscle Atrophy and Increases the Serum Levels of Active Form Myostatin in Rat Serum(Physiology)
- In Vivo Culture of IVF-Bovine Embryos in Murine Oviducts
- Substantial Evidence to Localize the Developmental Origin of Primordial Germ Cells in the Chicken
- Substantial Evidence to Localize the Developmental Origin of Primordial Germ Cells in the Chicken
- Transgenic Mice Overexpressing Foreign Whey Acidic Protein (WAP) Genes Driven by Metallothionein Promoter
- Effects of Transfected Human and Mouse SRY/Sry Genes upon the Expression of Hypothetical SRY-Cascade Genes in Cultured Mouse Sertoli Cell Line, TM-4
- Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11
- Expression of Matrix Metalloproteinase-11 (Stromelysin-3) and TIMP-1 Genes in the Placenta and the Uterus during Estrous Cycles and Gestation in the Mouse
- Inhibition of Mitogen Activated Protein Kinase Activity Induces Parthenogenetic Activation and Increases Cyclin B Accumulation during Porcine Oocyte Maturation
- Requirement of Mitogen-Activated Protein Kinase Activation for the Meiotic Resumption of Porcine Oocytes
- Investigation of the fate of Sry-expressing cells using an in vivo Cre/loxP system
- Aberrant Development of Mammary Glands, but Precocious Expression of β-casein in Transgenic Females Ubiquitously Expressing Whey Acidic Protein Transgene
- 胚から見た着床のメカニズム : トロフォブラスト浸潤とその制御
- 着床周辺期胚培養の諸問題 : 自律性と他律性
- Expression of Matrix Metalloproteinase-11 Genes in Placentae and Uteri of Shiba Goats (Capra hircus)
- Effect of Calcium lonophore A23187 upon the Rate of Leukotriene C_4 Production and the Cellular Morphology in Highly Purified Mouse Peritoneal Macrophages Cultured in vitro : Cell Biology
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Whey Acidic Protein (WAP) Depresses the Proliferation of Mouse (MMT) and Human (MCF-7) Mammary Tumor Cells
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- Role of Serum Myostatin during the Lactation Period
- プロジェステロンの前処置はエストロジェンにより誘導されるラット子宮上皮細胞でのc-ski mRNAの発現ならびに子宮上皮細胞の増殖を抑制する
- Expression and Possible Role of 20α-Hydroxysteroid Dehydrogenase in the Placenta of the Goat
- ヤギ20α-水酸化ステロイド脱水素酵素cDNAのクローニング
- Alteration in Anxiety with Relation to the Volume of the Locus Ceruleus in Progranulin-Deficient Mice
- Roles of Progranulin in Sexual Differentiation of the Developing Brain and Adult Neurogenesis
- In Vivo Gene Transfer of PPARγ Is Insufficient to Induce Adipogenesis in Skeletal Muscle(Physiology)
- Design of Hammerhead Ribozymes that Cleave Murine Sry mRNA in vitro and in vivo
- Skeletal Muscles of Transgenic Mice Expressing Human snoN, a Homologue of c-ski
- In vitro Cre/loxP system in cells from developing gonads : Investigation of the Sry promoter
- Survey of Homeobox Genes Expressed in Hemochorial Placentae of Mice (Mus musculus) and in Epitheliochorial/Syndesmochorial Placentae of Shiba Goats (Capra hircus var. Shiba)
- Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba, Goats (Capra hircus var Shiba)
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Age-Dependent Changes in Progranulin Expression in the Mouse Brain
- Satellite Cell Differentiation in Goat Skeletal Muscle Single Fiber Culture
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- Effects of Estrogen on Growth Hormone Pulsatility in Peripheral Blood and Neuropeptide Profiles in the Cerebrospinal Fluid of Goats
- An Attempt to Detect the Specific Antibody to Coccidia (Eimeria tenella) at the Infected Sites of Caecum in Immunized Chicken
- Production and Characterization of Transgenic Mice Expresssing a hGH Fusion Gene Driven by the Promoter of Mouse Whey Acidic Protein (mWAP) Putatively Specific to Mammary Gland
- Possible Involvement of Amiloride-Sensitive Na+/H+ Exchanger and/or Na+ Transport Systems in Hatching and the Trophoblast Spreading in Mouse Blastocysts Cultured in vitro
- Partial Purification of Gonadotropins from Chicken Anterior Pituitary and their Biological Activities
- Identification of Whey Acidic Protein (WAP) in Dog Milk
- Establishment of bipotent progenitor cell clone from rat skeletal muscle
- Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
- Dynamics of .BETA.ig-h3 mRNA Expression During Pregnancy in the Uterus and the Placenta of the Mouse: A Possible Regulatory Factor for Trophoblastic Invasion
- Analysis of the Promoter of Mutated Human Whey Acidic Protein (WAP) Gene
- Functions of the N-Glycans of Rat Leukemia Inhibitory Factor Expressed in Chinese Hamster Ovary Cells.