Analysis of the Promoter of Mutated Human Whey Acidic Protein (WAP) Gene
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概要
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Although whey acidic protein (WAP) has been identified in the milk of a range of species, it has been predicted that WAP is not secreted into human milk as a result of critical point mutations within the coding region. In the present study, we first investigated computationally the promoter region of mutated human WAP genes by comparing with those of other known WAP genes. Computational database analyses showed that the human WAP promoter region was highly conserved, as in other species with milk WAP. Next, we evaluated the activity of the human WAP promoter (2.6 kb) using a reporter gene assay. MCF-7 cells were stably transfected with the hWAP/hGH (human growth hormone) fusion gene, cultured on Matrigel, and treated with lactogenic hormones. Radioimmunoassay detected hGH in the culture medium, indicating that the human WAP promoter was responsible for the lactogenic hormones. The human WAP promoter was significantly more active in MCF-7 cells than the mouse WAP promoter (2.4 kb). The present results provide us with important information on the molecular evolution of milk protein genes.
- 日本繁殖生物学会の論文
著者
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Tojo Hideaki
Laboratory Of Animal Reproduction
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Yada Tetsushi
Human Genome Center Institute Of Medical Science University Of Tokyo
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Seki Mami
Laboratory Of Applied Genetics Graduate School Of Agriculture And Life Science University Of Tokyo
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Naito Kunihiko
Laboratory Of Applied Genetics Department Of Animal Resource Sciences Graduate School Of Agricultura
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TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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NUKUMI Naoko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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IWAMORI Tokuko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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