Insufficient Amount of Cdc2 and Continuous Activation of Wee1 B are the Cause of Meiotic Failure in Porcine Growing Oocytes
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概要
- 論文の詳細を見る
In mammals, growing oocytes with a diameter less than 80% of that of full-grown oocytes cannot start meiotic maturation, and their maturation promoting factor (MPF) cannot be activated by hormonal stimulation or isolation from follicles. The aim of the present study was to identify the key molecules responsible for meiotic failure of these growing oocytes (referred to as "small oocytes" in the present study). To this end, we altered the expression of the molecules involved in MPF activation in the small oocytes of pigs by injecting them with mRNA or antisense RNA (asRNA) and examined the effects on the meiotic ability of the small oocytes. Immunoblotting analyses revealed three defects in small oocytes compared with full-grown oocytes, an inactive mitogen activated protein kinase (MAPK) cascade, a failure of cyclin B synthesis and an insufficient amount of Cdc2. Injection with mRNAs of Mos, the uppermost molecule of the MAPK cascade, cyclin B1, cyclin B2 or Cdc2 into small porcine oocytes indicated directly and for the first time that the cause of meiotic failure of porcine small oocytes is an insufficient amount of Cdc2 rather than MAPK inactivation or failure of cyclin B synthesis. Next, in order to suppress Myt1 and Wee1B, which phosphorylates at inhibitory phosphorylation sites of Cdc2 and inactive MPF, we injected their asRNAs into the porcine small oocytes and found that the Wee1B asRNA significantly increased meiotic ability, whereas the Myt1 asRNA had no effect. When Cdc2 overexpression and suppression of Wee1B expression were simultaneously induced in the small oocytes of pigs, about 70% of the small oocytes resumed meiosis, and this rate was nearly comparable with that of the full-grown oocytes. These results strongly suggest that an insufficient amount of Cdc2 and continuous activation of Wee1 B are the cause of meiotic failure of small oocytes in pigs.
- 日本繁殖生物学会の論文
- 2009-10-01
著者
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Kano Kiyoshi
東京大学 農学生命科学研究科応用遺伝学
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Naito Kunihiko
東京大学 農学生命科学研究科応用遺伝学
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Kano Kiyoshi
Lab. Of Applied Genetics Graduate School Of Agriculture And Life Sciences The Univ. Of Tokyo
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Kano Kiyoshi
Laboratory Of Applied Genetics Graduate School Of Agriculture And Life Sciences The University Of To
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Naito Kunihiko
Lab. Of Applied Genetics Graduate School Of Agriculture And Life Sciences The Univ. Of Tokyo
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Naito Kunihiko
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Science University Of Tokyo
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Naito Kunihiko
Laboratory Of Applied Genetics Graduate School Of Agriculture And Life Sciences The University Of To
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Kano Kiyoshi
Laboratory Of Applied Genetics Department Of Animal Resource Science Graduate School Of Agriculture
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SHIMAOKA Takuma
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of
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NISHIMURA Takanori
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of
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Naito Kunihiko
Laboratory Of Applied Genetics Department Of Animal Resource Sciences Graduate School Of Agricultura
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Shimaoka Takuma
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
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Nishimura Takanori
Laboratory Of Applied Genetics Graduate School Of Agricultural And Life Sciences The University Of T
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