Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11
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概要
- 論文の詳細を見る
The prolactin (PRL)-responsive cell line, HC11, derived from normal mammary epithelial cell line COMMA-1D, expressed the long form of prolactin receptor (L-PRLR) mRNA, though the level was considerably lower than that in the mammary gland of the normal lactating mouse. HC11 cells expressed whey acidic protein (WAP) mRNA, when they were cultured to confluence and in vitro lactogenesis was induced by the addition of PRL, insulin and dexamethasone. The levels of PRL-induced WAP expression, as measured by reverse transcriptase mediated polymerase chain reaction (RT-PCR), increased in a dose-dependent manner, as PRL concentrations were raised from 0.05 μg/ml to 50 μg/ml. Treatment of HC11 cells with human growth hormone (hGH) caused increase in levels of WAP mRNA. In contrast to PRL, hGH showed its maximum effect at 0.5 μg/ml. Beyond this concentration, the effect of hGH was greatly reduced. These results suggest that hGH has a narrow optimal concentration range with regard to the stimulation of WAP expression in HC11 cells.
- 日本繁殖生物学会の論文
著者
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TANAKA Satoshi
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Tanaka S
Univ. Tokyo Tokyo Jpn
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Ishijima Junko
Laboratory Of Mammalian Genetics National Institute Of Genetics
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Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
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Kawamata Yuji
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource S
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SASAKI Masato
Zenyaku Kogyo Co., Ltd., 2-33-7, Oizumi-machi, Nerima-ku, Tokyo 177, Japan
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ENAMI Jumpei
Zenyaku Kogyo Co., Ltd., 2-33-7, Oizumi-machi, Nerima-ku, Tokyo 177, Japan
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