Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba Goats (Capra hircus var Shiba)
スポンサーリンク
概要
- 論文の詳細を見る
We cloned cDNA of βig-h3, a TGF-β responsive gene, from the caprine uterus by RT-PCR method combined with the 5- and 3- RACE. The caprine βig-h3 cDNA contains a single open reading frame (ORF) encoding a 683-amino-acid protein which is highly conserved among the three species so far examined, i. e., goats, mice and humans. The hydropathy plots of caprine p68βig-h3 (formerly βIG-H3) protein reveals that hydrophilic and hydrophobic domains are regularly repeated. The hydrophobic domain spanning from Met1-Ala23 reprents the signal peptide sequence indicating that caprine p68βig-h3, like in other species, is a secretory protein. On Northern blot analysis, the pregnant uterus exhibited a strong signal at 2.7 kb, while in the immature uterus and the placenta, signal levels were very low.
- 日本繁殖生物学会の論文
著者
-
TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
-
YAMANOUCHI Keitaro
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
-
Kim Y‐j
Univ. Tokyo Tokyo Jpn
-
Kim Yong-Jin
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
-
Uekita Takamasa
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
-
Sawasaki Toru
The Experimental Station for Bio-Animal Science, Graduate School of Agriculture and Life Sciences, T
-
Tachi Chikashi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Lif
-
Yamanouchi K
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
関連論文
- Involvement of 20α-Hydroxysteroid Dehydrogenase in the Maintenance of Pregnancy in Mice
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- 乳清タンパク質(WAP)を全身性に過剰発現するトランスジェニックマウスにおける乳腺の発達異常とβ-カゼインの早期発見
- Matrix metalloproteinases regulate mesonephric cell migration in developing XY gonads which correlates with the inhibition of tissue inhibitor of metalloproteinase-3 by sry
- ブタ卵に移植した卵丘細胞核へのサイクリンBとMAPキナーゼの局在
- 哺乳類卵子解析のための小規模ウエスタンブロッティング系「マイクロ・ウエスタンブロッティング」の確立
- ブタ卵成熟におけるMPF活性化に対する卵核胞の必要性の検討
- 乳清酸性タンパク質(WAP)はマウスおよびヒトの乳癌細胞(MMT,MCF-7)の増殖を抑制する
- マウス乳腺上皮細胞(EpH4/K6)の増殖能に及ぼす乳清酸性タンパク質の抑制作用
- Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba Goats (Capra hircus var Shiba)
- シバヤギ胎盤から樹立したクローン化培養細胞株CPF-1におけるテロメア長短縮の解析
- ウマ(サラブレッド)マイオスタチンcDNAのクローニングと血清中マイオスタチン前駆タンパク質の検出
- マウス(Mus Musculus)血液漿膜胎盤およびシバヤギ(Capra hircus var Shiba)上皮漿膜/結合組織漿膜胎盤におけるホメオボックス遺伝子発現の解析
- プロジェステロンの前処置はエストロジェンにより誘導されるラット子宮上皮細胞でのc-ski mRNAの発現ならびに子宮上皮細胞の増殖を抑制する
- ブタ卵の減数分裂再開にはMAPキナーゼの活性が必要である
- Novel Development of Mammary Glands in the Nursing Transgenic Mouse Ubiquitously Expressing WAP Gene
- Analysis of Gender Difference of Cardiac Risk Biomarkers Using hGH-Transgenic Mice
- Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes
- Expression of Membrane-Type 1 Matrix Metalloproteinase (MT1-MMP) mRNA in Trophoblast and Endometrial Epithelial Cell Populations of the Synepitheliochorial Placenta of Goats (Capra hircus)
- Possible role for the c-ski gene in the proliferation of myogenic cells in regenerating skeletal muscles of rats
- A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones
- In vitro Studies on PGC or PGC-Like Cells in Cultured Yolk Sac Cells and Embryonic Stem Cells of the Mouse
- Isolation of Satellite Cells from Equine Skeletal Muscle
- Skeletal Muscles of Transgenic Mice Expressing Human snoN, a Homologue of c-ski
- ウマ(サラブレッド種)各組織におけるc-ski遺伝子の発現について
- Cell-Cell Contract Down-Regulates Expression of Membrane Type Metalloproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line
- Transgenic Mice Overexpressing Foreign Whey Acidic Protein (WAP) Genes Driven by Metallothionein Promoter
- ウマの卵胞発育過程におけるインヒビン/アクチビンサブユニット,アロマターゼおよび3β-ヒデロキシステロイド脱水素酵素の発現
- Functions of the N-Glycans of Rat Leukemia Inhibitory Factor Expressed in Chinese Hamster Ovary Cells
- マウス卵管を利用した牛IVF胚の体内培養
- Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11
- Expression of Matrix Metralloproteinase-11 (Stromelysin-3) and TIMP-1 Genes in the Placenta and the Uterus during Estrous Cycles and Gestation in the Mouse
- Bacteriostatic Activity of Whey Acidic Protein (WAP)
- Expression of Ski in the Granulosa Cells of Atretic Follicles in the Rat Ovary
- Long Term Adrenal Insufficiency Induces Skeletal Muscle Atrophy and Increases the Serum Levels of Active Form Myostatin in Rat Serum(Physiology)
- In Vivo Culture of IVF-Bovine Embryos in Murine Oviducts
- Transgenic Mice Overexpressing Foreign Whey Acidic Protein (WAP) Genes Driven by Metallothionein Promoter
- Effects of Transfected Human and Mouse SRY/Sry Genes upon the Expression of Hypothetical SRY-Cascade Genes in Cultured Mouse Sertoli Cell Line, TM-4
- Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11
- Expression of Matrix Metalloproteinase-11 (Stromelysin-3) and TIMP-1 Genes in the Placenta and the Uterus during Estrous Cycles and Gestation in the Mouse
- Requirement of Mitogen-Activated Protein Kinase Activation for the Meiotic Resumption of Porcine Oocytes
- Investigation of the fate of Sry-expressing cells using an in vivo Cre/loxP system
- Aberrant Development of Mammary Glands, but Precocious Expression of β-casein in Transgenic Females Ubiquitously Expressing Whey Acidic Protein Transgene
- Expression of Matrix Metalloproteinase-11 Genes in Placentae and Uteri of Shiba Goats (Capra hircus)
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Whey Acidic Protein (WAP) Depresses the Proliferation of Mouse (MMT) and Human (MCF-7) Mammary Tumor Cells
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- Role of Serum Myostatin during the Lactation Period
- プロジェステロンの前処置はエストロジェンにより誘導されるラット子宮上皮細胞でのc-ski mRNAの発現ならびに子宮上皮細胞の増殖を抑制する
- Expression and Possible Role of 20α-Hydroxysteroid Dehydrogenase in the Placenta of the Goat
- ヤギ20α-水酸化ステロイド脱水素酵素cDNAのクローニング
- Alteration in Anxiety with Relation to the Volume of the Locus Ceruleus in Progranulin-Deficient Mice
- Roles of Progranulin in Sexual Differentiation of the Developing Brain and Adult Neurogenesis
- In Vivo Gene Transfer of PPARγ Is Insufficient to Induce Adipogenesis in Skeletal Muscle(Physiology)
- Design of Hammerhead Ribozymes that Cleave Murine Sry mRNA in vitro and in vivo
- Skeletal Muscles of Transgenic Mice Expressing Human snoN, a Homologue of c-ski
- In vitro Cre/loxP system in cells from developing gonads : Investigation of the Sry promoter
- Survey of Homeobox Genes Expressed in Hemochorial Placentae of Mice (Mus musculus) and in Epitheliochorial/Syndesmochorial Placentae of Shiba Goats (Capra hircus var. Shiba)
- Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba, Goats (Capra hircus var Shiba)
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Age-Dependent Changes in Progranulin Expression in the Mouse Brain
- Satellite Cell Differentiation in Goat Skeletal Muscle Single Fiber Culture
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- Effects of Estrogen on Growth Hormone Pulsatility in Peripheral Blood and Neuropeptide Profiles in the Cerebrospinal Fluid of Goats
- Possible Involvement of Amiloride-Sensitive Na+/H+ Exchanger and/or Na+ Transport Systems in Hatching and the Trophoblast Spreading in Mouse Blastocysts Cultured in vitro
- Identification of Whey Acidic Protein (WAP) in Dog Milk
- Establishment of bipotent progenitor cell clone from rat skeletal muscle
- Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
- Dynamics of .BETA.ig-h3 mRNA Expression During Pregnancy in the Uterus and the Placenta of the Mouse: A Possible Regulatory Factor for Trophoblastic Invasion