ブタ卵の減数分裂再開にはMAPキナーゼの活性が必要である
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概要
- 論文の詳細を見る
The requirement of mitogen-activated protein kinase (MAPK) for the initiation of meiotic maturation in porcine oocytes was examined using U0126, an inhibitor of MAPK activator. Immunoblot analysis and kinase assay showed that U0126 inhibited the phosphorylation and activation of MAPK incompletely in porcine oocytes. In this condition, maturation/M-phase promoting factor (MPF) activity was also inhibited and the rate of germinal vesicle breakdown (GVBD, 95%) was reduced to 42%. The classification of oocytes remaining at germinal vesicle stage (GV) and reached to second metaphase stage (M2) after 48h culture with U0126 revealed that GV oocytes had low MAPK and MPF activities while M2 oocytes had high activities in both kinases, suggesting the indispensability of MAPK activation for meiotic resumption of porcine oocytes. In mouse oocytes, however, the inhibition of MAPK activation by U0126 had no effect on GVBD nor MPF activation, consistent with previous reports using mos deficient mice. Additionally, we found that the inhibitory effects of U0126 needed the presence of cumulus cells because of the ineffectiveness of U0126 on denuded porcine oocytes. These results suggest that MAPK act as indispensable mediator of MPF activation and GVBD in porcine oocytes, like in Xenopus oocytes but not in mouse oocytes.
- 日本繁殖生物学会の論文
著者
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NAITO Kunihiko
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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TOJO Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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YAMANOUCHI Keitaro
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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GOTO Seitaro
Laboratory of Applied Genetics, Graduated School of Agricultural and Life Sciences, University of To
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SUGIURA Koji
Laboratory of Applied Genetics, Graduated School of Agricultural and Life Sciences, University of To
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KAGII Hideyuki
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultu
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IWAMORI Naoki
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultu
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杉浦 幸二
東京大学大学院・農学生命科学研究科・応用遺伝学教室
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内藤 邦彦
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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鍵井 英之
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultu
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杉浦 幸二
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultu
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OHASHI Satoshi
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultu
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