ブタ卵の第1減数分裂/第2減数分裂移行におけるCdk2活性の必要性
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概要
- 論文の詳細を見る
The meiotic progression of Xenopus oocytes has been suggested to depend on the activity of cyclin-dependent kinase 2 (Cdk2). We examined whether Cdk2 is involved in the regulation of mammalian oocyte meiosis by injecting porcine oocytes with anti-Cdk2 antibody. At first, the cross-reactivity of the anti-Cdk2 antibody with Cdc2 kinase was evaluated by immunoprecipitation and immunoblotting experiments using porcine granulosa cell extract, and no cross-reactivity with Cdc2 kinase was observed in the antibody used. In the anti-Cdk2 antibody-injected group, 50.7% of the oocytes were arrested in the second metaphase after 50 h of culture and this rate was significantly lower than those in the non-injected intact oocytes or the oocytes injected with mouse IgG (84.5% and 86.7%, respectively). Most of the other oocytes in the antibody-injected group formed a pronucleus without polar bodies or with only one polar body. The cyclin B1 amount in the antibody-injected and activated oocytes was dramatically decreased compared with that in the intact or mouse IgG-injected oocytes after 50 h of culture. These results suggest that Cdk2 is involved in the meiotic maturation of mammalian oocytes, and that the block of Cdk2 activity results in the failure of cyclin B1 accumulation and second meiosis induction.
- 日本繁殖生物学会の論文
著者
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杉浦 幸二
東京大学大学院・農学生命科学研究科・応用遺伝学教室
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杉浦 幸二
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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内藤 邦彦
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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東條 英昭
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Tojo Hideaki
Department Of Agricultural Chemistry The University Of Tokyo
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Naito Kunihiko
Department of Reproductive and Developmental Biology, The Institute of Medical Science, The Universi
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SUGIURA Koji
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Naito Kunihiko
Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers
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NAITO Kunihiko
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo
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TOJO Hideaki
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo
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