マウス卵管を利用した牛IVF胚の体内培養

概要

論文の詳細を見る
Although transfers of bovine embryos are mostly technically successful, the production of embryos for transfer remains a major problem. Manipulated embryos have been developed in the ligated oviducts of rabbits or sheep until the morulae or blastocyst stage, but these in vivo culture systems remain highly labor and cost intensive. The present paper describes the possible use of mouse oviducts without ligation in an in vivo culture system of in vitro fertilized (IVF) bovine embryos. IVF-derived 2-cell bovine embryos were transferred to the oviducts of pseudopregnant mice and were recovered by flushing the oviducts and the uteri at 5-7 days after the transfer. In the series of 22 experiments, a large proportion of the transferred 2-cell bovine embryos (180 of 307 embryos or 57.1%) were flushed from the oviducts, whereas a small number of embryos (6 embryos or 2.0%) that nearly degenerated were recovered from the uteri. In the recovered embryos, the mean percentage of the embryos that developed to morula or blastocysts was 71.1 ± 24.3%, whereas the ratio in in vitro culture was 33.8 ± 9.2%. The present results suggest that in vivo culture utilizing murine oviducts for the early stage bovine embryos may be available for efficiently obtaining late-stage embryos from farm animals.
日本繁殖生物学会の論文