Normal and Neoplastic Growth of Mammary Glands and Circulating Levels of Prolactin and Growth Hormone in Mouse Whey Acidic Protein Promoter/Human Growth Hormone (mWAP/hGH) Transgenic Mice

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Female and male hybrids between SHN females with high mammary tumor potentials and a male transgenic mouse expressing a high human growth hormone (hGH) fusion gene under the control of mouse whey acidic protein as a promoter were divided into hGH (+) and hGH (-) groups according to serum hGH levels at 50 days of age (111±16 ng/ml and 0.43±0.05 ng/ml in females and 51±4 ng/ml and 0.47±0.05 ng/ml in males, respectively). At both 2 and 4 months of age, female and male hGH (+) mice showed morphometrically a marked stimulation of mammary gland growth. In hGH (+) group, mammary gland contents of DNA and RNA in females and RNA content in males at 2 and 4 months were also significantly higher than in hGH (-) group. Furthermore, in males, thymidylate synthetase and thymidine kinase activities in the glands were higher in hGH (+) group at 4 month of age. In both females and males, mammary tumor incidence was enhanced in hGH (+) group. The estrous cycle of hGH (+) females had continued estrus. In both females and males, serum levels of mouse prolactin were higher in hGH (+) group than in hGH (-) group at 4 months. In serum mouse GH levels of both sexes, hGH (+) group was apparently lower and higher than hGH (-) group at 2 and 4 months of age, respectively. The possible role of hGH on normal and neoplastic mammary gland growth was discussed.
1993-12-15