Reproductive Phenotypes in Mice with Targeted Disruption of the 20α-Hydroxysteroid Dehydrogenase Gene
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概要
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In the corpus luteum of rats and mice, 20α-hydroxysteroid dehydrogenase (20α-HSD) catalyzes the conversion of progesterone to a biologically inactive metabolite, 20α-dihydroprogesterone (20α-OHP). The reduction of progesterone by 20 α-HSD is believed to be important for functional luteolysis in these rodent species. In addition to the corpus luteum, expression of 20α-HSD has been demonstrated in tissues such as the placenta, endometrial epithelia, and fetal skin, although the roles it plays in the latter tissues remain to be determined. To determine the contribution of 20α-HSD to functional luteolysis and to the rodent reproductive system more generally, we generated a strain of mice with targeted disruption of the 20 α-HSD gene. In the 20α-HSD-/-mice we obtained, which lacked the genomic region essential for catalytic reaction, neither 20α-HSD activity in the corpus luteum nor an increase in the serum concentrations of 20α-OHP during pseudopregnancy or pregnancy was detected. The durations of the estrous cycle, pseudopregnancy, and pregnancy were significantly prolonged in the 20α-HSD-/-mice, although the serum progesterone levels decreased to levels low enough for delivery of pups at term of pregnancy. In addition, the number of pups, especially live pups, was markedly decreased in the 20α-HSD-/- mice. These findings suggest that the role of 20α-HSD in functional luteolysis is relatively minor but that it is involved in the survival of newborn mice.
- 日本繁殖生物学会の論文
著者
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CHOI Jae-hyek
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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ISHIDA Maho
Department of Physiology, Interdisciplinary Graduate School of Medicine and Engineering, University
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MATSUWAKI Takashi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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YAMANOUCHI Keitaro
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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NISHIHARA Masugi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Sudo Katsuko
Center For Experimental Medicine Institute Of Medical Science University Of Tokyo
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IWAKURA Yoichiro
Center for Experimental Medicine, Institute of Medical Science, University of Tokyo
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SUZUKI Masatoshi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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HORAI Reiko
Center for Experimental Medicine, Institute of Medical Science, University of Tokyo
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Horai Reiko
Center For Experimental Medicine Institute Of Medical Science University Of Tokyo
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Matsuwaki Takashi
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Yamanouchi Keitaro
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
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Choi Jae-hyek
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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HIRABAYASHI Keiji
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Nishihara Masugi
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
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Ishida Maho
Department Of Physiology Interdisciplinary Graduate School Of Medicine And Engineering University Of
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Hirabayashi Keiji
Department Of Animal Production Ikuta Campus Meiji University
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Hayashi K
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro
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Suzuki Masatoshi
Department Of Cardiology Teikyo University School Of Medicine
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Iwakura Yoichiro
Center For Experimental Medicine And Systems Biology Institute Of Medical Science University Of Toky
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Ishida Maho
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Suzuki Masatoshi
Center For School Education Research Hyogo University Of Teacher Education
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Matsuwaki Takashi
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Horai Reiko
Center For Experimental Medicine Inst. Of Medical Sci. The Univ. Of Tokyo
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Iwakura Yoichiro
Center For Experimental Medicine And Systems Biology Inst. Of Medical Sci. The Univ. Of Tokyo
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Sudo Katsuko
Center For Experimental Medicine Inst. Of Medical Sci. The Univ. Of Tokyo
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NISHIHARA MASUGI
Department of Physiology, Yokohama City University School of Medicine
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Iwakura Yoichiro
Center for Experimental Medicine, Institute of Medical Science, The University of Tokyo
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SUDO Katsuko
Center for Experimental Medicine, Institute of Medical Science, The University of Tokyo
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NISHIHARA Masugi
Department of Physiology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
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HORAI Reiko
Center for Experimental Medicine, Institute of Medical Science, The University of Tokyo
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