マウス20α-水酸化ステロイド脱水素酵素遺伝子のクローニングとその妊娠期におけるmRNA発現
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概要
- 論文の詳細を見る
20α-Hydroxysteroid dehydrogenase (20α-HSD) converts progesterone to a biologically inactive steroid, 20α-dihydroprogesterone. To examine a potential role of 20α-HSD in tissues other than corpus luteum (CL) during pregnancy, mouse 20α-HSD cDNA was cloned and was used for the examination of its mRNA localization in the conceptus and uterus at different stages of gestation. Using probes based on the rat sequence, mouse 20α-HSD cDNA clone was isolated from a cDNA library prepared from ovaries of day 12 pseudopregnant mice. The mouse 20α-HSD cDNA was 1193 bp in length and encoded 323 amino acids with an estimated molecular weight of 37 kD. The mouse 20α-HSD was homologous to the rat 20α-HSD in nucleotide and amino acid sequences (both in 93% homology), and to other members of aldo-keto reductase superfamily at the cofactor (NADP(H)) binding site. By in situ hybridization, 20α-HSD mRNA was localized in endometrial epithelial cells on days 10, 15 and 18 of pregnancy, maternal placental endothelial cells on day 10, and fetal epidermal cells on day 15. During pseudopregnancy as well as early pregnancy, however, 20α-HSD mRNA was not detected in endometrial cells. Together, 20α-HSD mRNA was localized in cells that surround fetuses during the mid and late stages of gestation.
- 日本繁殖生物学会の論文
- 1999-10-01
著者
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ISHIDA Maho
Department of Physiology, Interdisciplinary Graduate School of Medicine and Engineering, University
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NISHIHARA Masugi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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石田 真帆
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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平林 啓司
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Chang K‐t
Univ. Tokyo Tokyo Jpn
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Chang K‐t
Korea Res. Inst. Of Bioscience And Biotechnology Daejon Kor
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TAKAHASHI Michio
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Matsuwaki Takashi
Dep. Of Veterinary Physiology Veterinary Medical Sci. The Univ. Of Tokyo
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CHANG Kyu-Tae
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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HIRABAYASHI Keiji
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Nishihara Masugi
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
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Nishihara Masugi
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Takahashi M
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Ishida Maho
Department Of Physiology Interdisciplinary Graduate School Of Medicine And Engineering University Of
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Hirabayashi Keiji
Department Of Animal Production Ikuta Campus Meiji University
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Hayashi K
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro
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Takahashi M
Ajinomoto Co. Tokyo Jpn
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Ishida Maho
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Takahashi Michio
Res. Inst. For Health Fundamentals Ajinomoto Co. Inc.
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Takahashi Michio
Department Of Internal Medicine Kitasato University School Of Medicine
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Nishihara Masugi
Department Of Veterinary Physiology The University Of Tokyo
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Takahashi Michio
Department Of Civil Engineering College Of Engineering Nihon University
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張 奎泰
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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NISHIHARA MASUGI
Department of Physiology, Yokohama City University School of Medicine
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Hayashi Katsuhiko
NTT Photonics Laboratories NTT Corporation
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NISHIHARA Masugi
Department of Physiology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
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TAKAHASHI MICHIO
Department of Chemical Engineering, University of Tokyo
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