Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
スポンサーリンク
概要
- 論文の詳細を見る
The Ski protein is implicated in the proliferation/differentiation of a variety of cells. We previously reported that the Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. However, granulosa cells cannot only undergo apoptosis but can alternatively differentiate into luteal cells. It is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to determine the localization of the Ski protein in the rat ovary during luteinization to examine if Ski might play a role in this process. In order to examine the Ski protein expression during the progression of luteinization, follicular growth was induced in immature female rats by administration of equine chorionic gonadotropin, and luteinization was induced by human chorionic gonadotropin treatment to mimic the luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in the preovulatory follicle, Ski protein expression was induced in response to the LH surge and was maintained after formation of the corpus luteum (CL). Although the Ski protein is absent from the granulosa cells of the preovulatory follicle, its mRNA (c-ski) was expressed, and the level of c-ski mRNA was unchanged even after the LH surge. The combined results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated posttranscriptionally.
- 日本繁殖生物学会の論文
著者
-
Matsuwaki Takashi
Dep. Of Veterinary Physiology Veterinary Medical Sci. The Univ. Of Tokyo
-
Yamanouchi Keitaro
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
-
Nishihara Masugi
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University
-
Kim Hyun
Department Of Agricultural Bio-resources National Academy Of Agricultural Science Rural Development
-
Matsuwaki Takashi
Department Of Veterinary Physiology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
関連論文
- Gene cloning and enzymatic properties of hyperthermostable β-glycosidase from Thermus thermophilus HJ6(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Long-Term Clinical Course of Patients With Isolated Myocardial Bridge
- Involvement of 20α-Hydroxysteroid Dehydrogenase in the Maintenance of Pregnancy in Mice
- Molecular Characteristics of Bovine Serum Albumin-Dextran Conjugates
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- Retinyl retinoate induces hyaluronan production and less irritation than other retinoids
- Matrix metalloproteinases regulate mesonephric cell migration in developing XY gonads which correlates with the inhibition of tissue inhibitor of metalloproteinase-3 by sry
- 哺乳類卵子解析のための小規模ウエスタンブロッティング系「マイクロ・ウエスタンブロッティング」の確立
- ブタ卵成熟におけるMPF活性化に対する卵核胞の必要性の検討
- Circadian modulation of hepatic transcriptome in transgenic rats expressing human growth hormone
- Effects of Heat-Processed Ginseng and Its Active Component Ginsenoside 20(S)-Rg3 on the Progression of Renal Damage and Dysfunction in Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty Rats
- ヤギ胎盤における20α-水酸化ステロイド脱水素酵素の発現とその役割
- リポソーム・ペプチド・DNA複合体は精子ベクター法による遺伝子導入効率を向上する
- 新生ラット視床下部におけるエストロジェン受容体、アンドロジェン受容体およびアロマターゼの遺伝子発現に対するエストロジェン処置の影響
- PJ-404 Prognosis of Functional Mitral Valve Regurgitation after Aortic Valve Replacement(Valvular herat disease/Pericarditis/Cardiac tumor-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-349 Evaluation of Appropriate Bypass Graft Length in Coronary Artery Bypass Grafting with 64-row Multidetector Computed Tomography(CT/DSA-09, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Clinical course of non-severe aplastic anemia in adults
- Substantial role of locus coeruleus-noradrenergic activation and capsaicin-insensitive primary afferent fibers in bee venom's anti-inflammatory effect
- 新生ラット視床下部における性ステロイド依存性遺伝子の発現解析
- 性腺刺激ホルモン放出ホルモンニューロンに緑色蛍光タンパク質を発現するトランスジェニックラットの作出
- シバヤギにおけるプロスタグランジンF2αの繰返し投与による不完全性周期の誘起
- Interleukin 1 Receptor Antagonist Is Important in the Suppression of Neointima Formation after Injury
- TED-AJ03-292 FLOW AND ABSORPTION CHARACTERISTICS OF AMMONIA-WATER MIXTURE IN A SLUG FLOW ABSORBER AT LOW SOLUTION FLOW RATE
- Ectopically Erupting First Permanent Molar in Chronic Myelogenous Leukemia
- Genetic Characteristics of Inbred Wuzhishan Miniature Pigs, a Native Chinese Breed
- Regulation of growth hormone pulsatility in female goats : modulation by sex steroids and involvement of neuropeptide Y
- Both PPARγ and C/EBPα are Sufficient to Induce Transdifferentiation of Goat Fetal Myoblasts into Adipocytes
- A Case of Hidroacanthoma Simplex
- A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones
- In vitro Studies on PGC or PGC-Like Cells in Cultured Yolk Sac Cells and Embryonic Stem Cells of the Mouse
- Isolation of Satellite Cells from Equine Skeletal Muscle
- ウマ(サラブレッド種)各組織におけるc-ski遺伝子の発現について
- Cell-Cell Contract Down-Regulates Expression of Membrane Type Metalloproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line
- Formation of p-Type Hg_Cd_Te Epilayers Grown on CdTe Buffer Layers on GaAs (211) B due to In Situ Thermal Annealing
- OE-363 Deficiency of Interleukin-1 Receptor Antagonist Induces Aortic Valve Stenosis in BALB/c Mice(Valvular heart disease/Pericarditis/Cardiac tumor-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- 3 Deficiency of lnterleukin-1 Receptor Antagonist Increases the Atherosclerosis in Apolipoprotein E-Deficient Mice(Young Investigator's Award Finalists Lectures (YIA))(Special Program)
- Possible Mechanisms for the Testis-Mediated Gene Transfer as a New Method for Producing Transgenic Animals
- Production of Transgenic Rats and Mice by the Testis-Mediated Gene Transfer
- hGH Transgenic Rats Expressing Severe Obesity and Effect of Treatment with hGH in a Pulsatile Manner
- Fresh and pickled vegetable consumption and gastric cancer in Japanese and Korean populations : A meta-analysis of observational studies
- Abnormal T cell activation caused by the imbalance of the IL-1/IL-1R antagonist system is responsible for the development of experimental autoimmune encephalomyelitis
- Beam Smoothing in a Passive Q-Switched Laser with an Additional Stimulated Brillouin Scattering Output Coupler
- Multiple gastrointestinal stromal tumors with a germline c-kit mutation
- Tumor Necrosis Factor-α Mediates Endotoxin Induced Suppression of Gonadotropin-Releasing Hormone Pulse Generator Activity in the Rat
- Reproductive Phenotypes in Mice with Targeted Disruption of the 20α-Hydroxysteroid Dehydrogenase Gene
- Characterization of GH Pulsatility in Male Shiba Goats : Effects of Postpubertal Castration and KP102
- Expression of Ovarian 20α-Hydroxysteroid Dehydrogenase in Rat Thymus
- EXPRESSION OF 20α - HYDROXYSTEROID DEHYDROGENASE IN RAT THYMUS
- ROLE OF GRANULIN THE PROCESS OF SEXUAL DIFFERENTIATION OF THE RAT BRAIN
- Hypothalamic Gonadotropin-Releasing Hormone Gene Expression during Rat Estrous Cycle
- ELECTROPHYSIOLOGICAL STUDIES ON THE HYPOTHALAMIC GnRH PULSE GENERATOR
- マウス20α-水酸化ステロイド脱水素酵素ゲノムDNAのクローニングとその遺伝子座の決定
- Prolactin Expresses Differential Effects on Apoptotic Cell Death of Luteal Cells In Vivo and In Vitro
- Splenic Macrophages Can Modify Steroidogenesis of Leydig Cells
- Expression of Ski in the Granulosa Cells of Atretic Follicles in the Rat Ovary
- Long Term Adrenal Insufficiency Induces Skeletal Muscle Atrophy and Increases the Serum Levels of Active Form Myostatin in Rat Serum(Physiology)
- In Vivo Culture of IVF-Bovine Embryos in Murine Oviducts
- Effects of Transfected Human and Mouse SRY/Sry Genes upon the Expression of Hypothetical SRY-Cascade Genes in Cultured Mouse Sertoli Cell Line, TM-4
- Requirement of Mitogen-Activated Protein Kinase Activation for the Meiotic Resumption of Porcine Oocytes
- Investigation of the fate of Sry-expressing cells using an in vivo Cre/loxP system
- S-V-4 Adipogenic differentiation of skeletal muscle progenitor/stem cells(The Proceedings of the 17th Annual Meetings of Japan Society of Exercise and Sports Physiology Jury 25-26, (Tokyo))
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- Role of Serum Myostatin during the Lactation Period
- プロジェステロンの前処置はエストロジェンにより誘導されるラット子宮上皮細胞でのc-ski mRNAの発現ならびに子宮上皮細胞の増殖を抑制する
- Expression and Possible Role of 20α-Hydroxysteroid Dehydrogenase in the Placenta of the Goat
- ヤギ20α-水酸化ステロイド脱水素酵素cDNAのクローニング
- Alteration in Anxiety with Relation to the Volume of the Locus Ceruleus in Progranulin-Deficient Mice
- Roles of Progranulin in Sexual Differentiation of the Developing Brain and Adult Neurogenesis
- In Vivo Gene Transfer of PPARγ Is Insufficient to Induce Adipogenesis in Skeletal Muscle(Physiology)
- ラット卵巣におけるグラニュリン前駆体蛋白(アクログラニン)の卵子特異的な発現
- Sex and circadian modulatory effects on rat liver as assessed by transcriptome analyses
- 周生期ラットにおけるメトキシクロール暴露が成熟後の生殖機能に与える影響
- Sex-Related Differences in Gene Expression in Neonatal Rat Hypothalamus Assessed by cDNA Microarray Analysis
- Skeletal Muscles of Transgenic Mice Expressing Human snoN, a Homologue of c-ski
- Morphological Studies on Testicular Development in the Horse
- In vitro Cre/loxP system in cells from developing gonads : Investigation of the Sry promoter
- Survey of Homeobox Genes Expressed in Hemochorial Placentae of Mice (Mus musculus) and in Epitheliochorial/Syndesmochorial Placentae of Shiba Goats (Capra hircus var. Shiba)
- Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba, Goats (Capra hircus var Shiba)
- マウス20α-水酸化ステロイド脱水素酵素遺伝子のクローニングとその妊娠期におけるmRNA発現
- Inhibin α-Subunit mRNA Is Expressed in the Corpus Luteum of the Pregnant Mare
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Involvement of Granulin in Estrogen-Induced Neurogenesis in the Adult Rat Hippocampus
- Age-Dependent Changes in Progranulin Expression in the Mouse Brain
- Involvement of Prostaglandins in Suppression of Gonadotropin-Releasing Hormone Pulse Generator Activity by Tumor Necrosis Factor-α
- Satellite Cell Differentiation in Goat Skeletal Muscle Single Fiber Culture
- Myogenic and Adipogenic Properties of Goat Skeletal Muscle Stem Cells
- The Effects of GABA on Embryonic Gonadotropin-Releasing Hormone Neurons in Rat Hypothalamic Primary Culture
- Effects of Estrogen on Growth Hormone Pulsatility in Peripheral Blood and Neuropeptide Profiles in the Cerebrospinal Fluid of Goats
- Blockade of the Preovulatory Release of Gonadotropins and Prolactin by Spinal Transections in Female Rats
- Effect of γA-Aminobutyric Acid on Excitability of Tubero-Infundibular Neurons in Rat Hypothalamic Slices
- Androgen Distribution in Male Rats
- Changes in Ratios of Phagocytotic Macrophages in the Splenic Adherent Cells during Estrous Cycle and Pseudopregnancy in Rats
- Identification of Whey Acidic Protein (WAP) in Dog Milk
- Establishment of bipotent progenitor cell clone from rat skeletal muscle
- In Vivo Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle
- Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
- Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
- Possible Involvement of Neuropeptide Y in Photo-Induced Suppression of Growth Hormone Pulses
- Differential Roles of Cyclooxygenase-2-Related Signaling in Regulating Hypothalamic Neuronal Activity under Various Acute Stresses
- Involvement of Activin and Inhibin in the Regulation of Food and Water Intake in the Rat.