Intratesticular Tissue Fluid (ITF) for the Micro-Bioassay of LH
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概要
- 論文の詳細を見る
Intratesticular tissue fluid (ITF) was obtained by centrifugation of the decapsulated testis of the adult rat. Testosterone level in ITF was increased by direct injection of LH into the testis and was lowered by anti-LH serum. Based on the result of previous reports with testosterone in testicular vein blood, a simplification of the LH bioassay without imparing sensitivity and accuracy was undertaken using ITF as the material for testosterone measurement. Dose-response relationship between injected LH and testosterone concentration in ITF was observed. The sensitivity of the assay was 5 ng for LH (NIH-LH-S15). FSH (NIH-FSH-S10) and prolactin (NIH-P-S11) had no detectable activity on testosterone production. This assay method depending on biological activities of LH will be helpful for unbiased evaluation of the result obtained by radioimmunoassay.
- 社団法人 日本内分泌学会の論文
著者
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Shiota Kunio
Department Of Veterinary Biochemistry Faculty Of Agriculture The University Of Tokyo
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TAKAHASHI Michio
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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SUZUKI YOSHISUKE
Department of Veterinary Physiology, Faculty of Agriculture, University of Tokyo
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Takahashi Michio
Department Of Internal Medicine Kitasato University School Of Medicine
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Takahashi Michio
Department Of Civil Engineering College Of Engineering Nihon University
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SUZUKI YOSHISUKE
Department of Veterinary Physiology, Faculty of Agriculture University of Tokyo
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Shirota Kunio
Department of Veterinary Physiology, Faculty of Agriculture, University of Tokyo
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