Characterization of Rat Mid-Pregnancy-Specific Placental Lactogen Produced by Baculovirus / Insect Cell Expression System
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概要
- 論文の詳細を見る
The placenta produces prolactin (PRL)- or growth hormone-like hormones known as placental lactogens (PLs) which play roles in the maternal endocrine system and fetal growth development. We previously cloned a cDNA encoding a novel rat PL, PL-I mosaic (PL-Im), which is specifically expressed at mid-pregnancy. In this study, the biological activity of PL-Im was evaluated by producing the recombinant protein (recPL-Im) by means of a baculovirus/insect cell expression system. RecPL-Im demonstrated binding activity for PRL receptor in a radioreceptor assay (RRA) system with rat liver membrane fraction and 125I-ovine PRL (oPRL). In addition, the activity of recPL-Im is comparable to oPRL in the Nb2 cell proliferation assay. Interestingly, recPL-Im stimulated progesterone secretion in a concentration-dependent manner in a primary culture of luteal cells from pregnant rat, whereas oPRL had no effect on luteal cells. The luteotropic function of recPL-Im was accompanied by suppression of the secretion of 20α-dihydroprogesterone (20α-OHP) in the culture. PL-Im therefore has ability to bind PRL-receptor and express PRL-like activity. In addition, recPL-Im has luteotropic hormone (LTH) activity and its activity is qualitatively different from that of PRL.
- 日本繁殖生物学会の論文
- 1999-02-01
著者
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小川 智久
Graduate School Of Life Sciences Tohoku University
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Ogawa T
Animal Resource Science/veterinary Medical Science University Of Tokyo
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Shiota Kunio
Laboratory Of Cellular Biochemistry Veterinary Medical Science/animal Resource Science The Universit
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Shiota Kunio
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Shiota Kunio
Department Of Veterinary Biochemistry Faculty Of Agriculture The University Of Tokyo
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Shiota K
Univ. Tokyo Tokyo Jpn
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HIROSAWA TAKAMORI
Animal Resource Science/Veterinary Medical Science, University of Tokyo
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MATSUURA Yoshiharu
Department of Virology II, National Institute of Health
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TANAKA Satoshi
Animal Resource Science/Veterinary Medical Science, University of Tokyo
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OGAWA Tomoya
Animal Resource Science/Veterinary Medical Science, University of Tokyo
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SHIOTA Kunio
Animal Resource Science/Veterinary Medical Science, University of Tokyo
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Matsuura Yoshiharu
Department Of Virology Ii National Institute Of Health
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Matsuura Yoshiharu
Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University
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Sasai Ken
Laboratory Of Cellular Biochemistry The University Of Tokyo
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Ogawa T
Laboratory Of Cellular Biochemistry Veterinary Medical Science/animal Resource Science The Universit
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Shiota K
Animal Resource Science/veterinary Medical Science University Of Tokyo
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Shiota Kunio
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers
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Hirosawa Takamori
Animal Resource Science/veterinary Medical Science University Of Tokyo
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Tanaka S
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers
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Hirosawa-Takamori Mitsuko
Animal Resource Science/Veterinary Medical Science, University of Tokyo
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