Acid Hydrolysis of Protein in a Microcapillary Tube for the Recovery of Tryptophan
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概要
- 論文の詳細を見る
The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 μl in volume) with 6 M HCl containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145 °C for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
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小川 智久
Graduate School Of Life Sciences Tohoku University
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Ogawa T
Animal Resource Science/veterinary Medical Science University Of Tokyo
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MURAMOTO Koji
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
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村本 光二
Lab. Of Functional Biomolecules Graduate School Of Life Sciences Tohoku Univ.
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OGAWA Tomohisa
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University
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ADEBIYI Abayomi
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University
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JIN Dong-Hao
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University
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Jin Dong-hao
Department Of Biomolecular Science Graduate School Of Life Sciences Tohoku University
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Muramoto Koji
Laboratory Of Applied Phytochemistry Tohoku University Graduate School Of Agricultural Science
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Muramoto Koji
Graduate School Of Agricultural Science Tohoku University
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Muramoto Koji
Department Biomolecular Science Tohoku University
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Adebiyi Abayomi
Department Of Biomolecular Science Graduate School Of Life Sciences Tohoku University
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Muramoto K
Graduate School Of Agricultural Science Tohoku University
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Ogawa T
Laboratory Of Cellular Biochemistry Veterinary Medical Science/animal Resource Science The Universit
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Jin Dong-Hao
Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University
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