Purification and Some Properties of High-molecular-weight Xylanases, the Xylanases 4 and 5 of Aeromonas caviae W-61
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概要
- 論文の詳細を見る
Aeromonas caviae W-61 produces multiple extracellular xylanases, the xylanases 1,2,3,4,and 5 [Nguyen, V. D. et al., Biosci. Biotechnol. Biochem., 56,1708-1712 (1993)]. Here we purified and characterized high-molecular-weight xylanases, the xylanases 4 and 5 from the culture fluids of the bacterium. The purified xylanases 4 and 5,which had molecular masses of 120 and 140 kDa, respectively, were endo-β-1,4-xylanases with similar enzymatic properties except for transxylosidase activity. The xylanase 4 showed a prominent transxylosidase activity when xylotriose and xylotetraose were used as the substrates, while the xylanase 5 had little transxylosidase activity under the same conditions. Protein sequencing indicated that the xylanase 4 was a C-terminally-truncated xylanase 5,suggesting that the C-terminal truncation of the xylanase 5 may endow the enzyme with transxylosidase activity.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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Muramoto Koji
Laboratory of Functional Biomolecules, Graduate School of Life Sciences, Tohoku University
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Kawai Yasushi
東北大学 農学研究科
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村本 光二
Lab. Of Functional Biomolecules Graduate School Of Life Sciences Tohoku Univ.
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Kawai Yoshiyuki
Tokyo Dietitian Academy
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Muramoto Koji
Laboratory Of Applied Phytochemistry Tohoku University Graduate School Of Agricultural Science
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Kawai Y
Kyoto Univ. Kyoto
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TOMITA Toshio
Laboratory of the Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of
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ROY Narayan
Laboratory of Applied Microbiology Tohoku University Graduate School of Agricultural Science
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OKAI Naoko
Laboratory of Applied Microbiology Tohoku University Graduate School of Agricultural Science
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Tomita T
Showa Sangyo Co. Ltd.
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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神尾 好是
東北大学
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