Identification of the Amino Acid Residues Conferring Substrate Specificity upon Selenomonas ruminantium Lysine Decarboxylase

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概要

• 論文の詳細を見る

• Lysine decarboxylase (LDC, EC 4.1.1.18) from Selenomonas ruminantium has decarboxylating activities towards both L-lysine and L-ornithine with similar K_m and V_<max■> Here, we identified four amino acid residues that confer substrate specificity upon S. ruminantium LDC and that are located in its catalytic domain. We have succeeded in converting S. ruminantium LDC to an enzyme with a preference in decarboxylating activity for L-ornithine when the four-residue of LDC were replaced by the corresponding residues of mouse ornithine decarboxylase (EC 4.1.1.17).

• 社団法人日本農芸化学会の論文
• 1999-10-23

著者

• Kawai Yasushi

  東北大学 農学研究科

• TOMITA Toshio

  Laboratory of the Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of

• TAKATSUKA Yumiko

  Laboratories of the Applied Microbiology Graduate School of Agricultural Science Tohoku University

• Tomita T

  Showa Sangyo Co. Ltd.

• Kamio Yoshiyuki

  Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult

• Takatsuka Y

  Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult

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