Purification and Characterization of Maltose Phosphorylase from Bacillus sp. RK-1(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Bacillus sp. RK-1 was isolated as a bacterium that produced maltose phosphorylase (MPase) in the culture supernatant. Screening was done from among about 400 isolates that could grow at 55℃ in a medium containing maltose as the sole carbon source. The enzyme was purified to an electrophoretically homogeneous state and some properties were investigated. The M^r of the enzyme was estimated to be 170 kDa by gel filtration and 88.5 kDa by SDS-PAGE, suggesting that it consisted of two identical subunits. The enzyme showed optimum activity around pH 6.0-7.0 and the optimum temperature was about 65℃. The enzyme was stable in the range of pH 5.5-8.0 after keeping it at 4℃ for 24 h and retained the activity up to about 55℃ after keeping it for 15 min. This is the first report about an MPase that could be produced in the culture supernatant. Furthermore, these investigations showed that this MPase is one of the most thermostable ones reported so far.
- 社団法人日本農芸化学会の論文
- 2001-12-23
著者
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Inoue Yuichi
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Fukui Fumio
Showa Sangyo Co. Ltd.
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Inoue Y
Showa Sangyo Co. Ltd.
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Inoue Y
Solar Energy Research Group Institute For Physical And Chemical Research
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Inoue Yorinao
Photosynthesis Research Lab.
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INOUE Yasushi
Showa Sangyo Co., Ltd.
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TOMITA Tetsuji
Showa Sangyo Co., Ltd.
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ISHII Keiko
Showa Sangyo Co., Ltd.,
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Yoshino Ichiro
The Department Of Surgery Ii Faculty Of Medicine University Of Occupational And Environmental Health
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Ishii Keiko
Department Of Laboratory Medicine Shinshu University School Of Medicine Nagano
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Tomita T
Showa Sangyo Co. Ltd.
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Ishii Keiko
Showa Sangyo Co. Ltd.
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