Novel Characteristics of Selenomonas ruminantium Lysine Decarboxylase Capable of Decarboxylating Both L-Lysine and L-Ornithine
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概要
- 論文の詳細を見る
Lysine decarboxylase (LDC ; EC 4.1.1.18) of Selenomonas ruminantium is a constitutive enzyme and is involved in the synthesis of cadaverine, which is an essential constituent of the peptidoglycan for normal cell growth. We purified the S. ruminantium LDC by an improved method including hydrophobic chromatography and studied the fine characteristics of the enzyme. Kinetic study of LDC showed that S. ruminanitum LDC decarboxylated both L-lysine and L-ornithine with similar K_m and the decarboxylase activities towards both substrates were competitively and irreversibly inhibited by DL-α-difluoromethylornithine, which is a specific inhibitor of ornithine decarboxylase (EC 4.1.1.17). We also showed a drastic descent of LDC activity owing to the degradation of LDC at entry into the stationary phase of cell growth.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Kawai Yasushi
東北大学 農学研究科
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村本 光二
Lab. Of Functional Biomolecules Graduate School Of Life Sciences Tohoku Univ.
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Muramoto Koji
Laboratory Of Applied Phytochemistry Tohoku University Graduate School Of Agricultural Science
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TOMITA Toshio
Laboratory of the Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of
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TAKATSUKA Yumiko
Laboratories of the Applied Microbiology Graduate School of Agricultural Science Tohoku University
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ONODA Motoko
Laboratories of the Applied Microbiology Graduate School of Agricultural Science Tohoku University
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SUGIYAMA Takeyoshi
Applied Bioorganic Chemistry Graduate School of Agricultural Science Tohoku University
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MURAMOTO Koji
Applied Phytochemistry Graduate School of Agricultural Science Tohoku University
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Tomita T
Showa Sangyo Co. Ltd.
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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Takatsuka Y
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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