Further Study on the Two Pivotal Parts of Hlg2 for the Full Hemolytic Activity of Staphylococcal γ-Hemolysin
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概要
- 論文の詳細を見る
Staphylococcal γ-hemolysin consists of LukF of 34 kDa and Hlg2 (or HγII) of 32 kDa, which cooperatively lyse human and rabbit erythrocytes. Our previous data showed that the 5-residue segment K^<23>R^<24>L^<25>A^<26>I^<27> of Hlg2 is pivotal for the hemolytic activity [Nariya, H. and Kamio, Y., Biosci. Biotechnol. Biochem., 59,1603-1604 (1997)]. Here, we identify an additional amino acid residue in Hlg2 necessary for the full γ-hemolysin activity by measuring the toxin activity of Hlg2 mutants in the presence of LukF. The data obtained showed that Arg^<217> of Hlg2 is an additional pivotal amino acid residue besides the KRLAI segment for the full Hlg2-specific function in γ-hemolysin. We also report evidence that the Hlg2 mutants showing a low or null hemolytic activity in the presence of LukF towards human erythrocytes had low or no binding activity to the cells, resulting in failure of formation of the ring-shaped pore-forming complex on the erythrocytes.
- 社団法人日本農芸化学会の論文
- 1998-09-23
著者
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Kawai Yasushi
東北大学 農学研究科
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SUGAWARA NORIKO
Department of Pediatrics, Tohoku University Graduate School of Medicine
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金子 淳
東北大学大学院農学研究科応用微生物学分野
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Kaneko Jun
Tohoku Univ. Sendai Jpn
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YOKOTA Kenji
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry
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Yokota K
Department Of Bacteriology Okayama University Graduate School Of Medicine And Dentistry
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Yokota K
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Yokota Kenji
Department Of Bacteriology Okayama University Graduate School Of Medicine And Dentistry
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金子 淳
東北大農化
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Kaneko Jun
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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Kaneko Jun
Department Of Applied Microbiology Graduate School Of Agricultural Science Tohoku University
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NARIYA Hirofumi
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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TOMITA Toshio
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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Nariya H
Tohoku Univ. Sendai Jpn
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Nariya Hirofumi
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Yokota Kenji
Okayama Univ. Graduate School Of Medicine And Dentistry Okayama Jpn
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Tomita T
Showa Sangyo Co. Ltd.
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Tomita Toshio
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Sugawara N
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Sugawara Noriko
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Kamio Yoshiyuki
Department Of Agricultural Chemistry Tohoku University
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Yokota Kenji
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic
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Oguma Keiji
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic
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Yokota Kenji
Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agric
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