A New Assay Using Surface Plasmon Resonance (SPR) to Determine Binding of the Lactobacillus acidophilus Group to Human Colonic Mucin

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概要

• 論文の詳細を見る

• A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.

• 社団法人 日本農芸化学会の論文
• 2004-05-23

著者

• SAITO Kazuya

  Department of Chemistry, Graduate School of Pure and Applied Sciences, University of Tsukuba

• Yasushi Kawai

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Kawai Yasushi

  東北大学 農学研究科

• HORII Akira

  Department of Molecular Pathology, Tohoku University School of Medicine

• Kawai Yoshiyuki

  Tokyo Dietitian Academy

• KITAZAWA Haruki

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• SAITO Tadao

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• Saito T

  Univ. Tokyo Jpn

• Kawai Y

  Kyoto Univ. Kyoto

• KAWAI Yasushi

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• FUJITANI Kenji

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• UCHIDA Hideaki

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• SHIIBA Kenichi

  Department of Gastrointestinal and Colorectal Surgery, Tohoku University School of Medicine

• Uchida Hideaki

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Shiiba Kenichi

  Department Of Gastrointestinal And Colorectal Surgery Tohoku University School Of Medicine

• Fujitani Kenji

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Horii A

  Department Of Molecular Pathology Tohoku University School Of Medicine

• Kamio Yoshiyuki

  Laboratory Of Applied Microbiology Tohoku University Graduate School Of Agricultural Science

• Saito Kazuya

  Department Of Aeronautics And Astronautics Graduate School Kyoto University

• Kawai Y

  Faculty Of Veterinary And Animal Science Nippon Veterinary And Animal Science University

• Shiiba K

  Nisshin Flour Milling Inc. Ibaraki Jpn

• Kamio Yoshiyuki

  Laboratory Of Applied Microbiology Department Of Molecular And Cell Bioloby Graduate School Of Agric

• Katoh S

  Department Of Gastrointestinal And Colorectal Surgery Tohoku University School Of Medicine

• Kitazawa Haruki

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Saito Tadao

  Food Science And Technology Institute Morinaga Milk Industry Co. Ltd.

• Horii Akira

  Department Of Materials Science And Technology Science University Of Tokyo

• 神尾 好是

  東北大学

• Kawai Y

  Faculty Of Fisheries Sciences Hokkaido University

• 北澤 春樹

  国立大学法人東北大学大学院農学研究科

• Saito Tadao

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

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