Purification and Characterization of α-Keto Ester Reductases from Bakers' Yeast

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概要

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• Three α-keto ester reductases (yeast keto ester reductase, YKER-II, -IV, -V) were purified from bakers'yeast. YKER-II, -IV, and -V are dimeric, monomeric, and dimeric enzymes, respectively, and molecular masses are estimated to be 58, 31-39, and 83kDa, respectively, based on gel filtration and SDS-polyacrylamide electrophoresis. Kinetic parameters and stereoselectivities in reduction of α-keto esters have been measured. YKER-IV contributes mainly to reduction by bakers' yeast at low substrate concentrations, and is useful for synthetic purposes.

• 社団法人日本農芸化学会の論文
• 1994-12-23

著者

• 中島 伸佳

  岡山県立大学短期大学部

• 中島 伸佳

  岡山県立短大

• NAKAJIMA Nobuyoshi

  Department of Nutritional Science, Okayama Prefectural University

• NAKAMURA Kaoru

  Institute for Chemical Research, Kyoto University

• KONDO Shin-ichi

  Institute for Chemical Research, Kyoto University

• OHNO Atsuyoshi

  Institute for Chemical Research, Kyoto University

• Yasushi Kawai

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Kawai Yasushi

  Institute For Chemical Research Kyoto University

• 中村 薫

  京大 化研

• Kawai Yoshiyuki

  Tokyo Dietitian Academy

• Kawai Y

  Kyoto Univ. Kyoto

• Ohno Atsuyoshi

  Institute For Chemical Research Kyoto Univ.

• Kondo S

  Ntt Opto‐electronics Lab. Atsugi‐shi Jpn

• Kondo S

  Institute For Chemical Research Kyoto University

• Kondo Shin-ichi

  Research Center For Development Of Far-infrared Region Fukui University

• Nakajima Nobuyoshi

  Graduate School Of Health And Welfare Okayama Prefectural University

• Nakajima Nobuyoshi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Ohno A

  Institute For Chemical Research Kyoto University

• Kondo S

  Department Of Materials Science And Engineering Nagasaki University

• Nagahama Kazuhiro

  Department Of Applied Microbial Technology The Kumanoto Institute Of Technology

• Atsuyoshi Ohno

  Institute For Chemical Research Kyoto University

• Nakamura Kaoru

  Institute For Chemical Research Kyoto University

• Nakamura Kahoru

  Department Of Nutrition Tokyo University Of Agriculture

• Kawai Y

  Faculty Of Fisheries Sciences Hokkaido University

• Nakajima Nobuyoshi

  Department of Food and Nutrition, Okayama Prefectural Junior College

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