Enzymatic Production of β-Thujaplicin 2-O-β-D-Glucoside in a Membrane Reactor Using an Insoluble UDP-Glucosyltransferase Fraction from Cultured Cells of Eucalyptus perriniana
スポンサーリンク
概要
- 論文の詳細を見る
Four UDP-glucosyltransfease (GTF) fractions were separated from the cultured cells of Eucalyptus perriniana, all of which exclusively produced β-thujaplicin 2-O-β-D-glucoside from β-thujaplicin (hinokitiol) in the presence of UDP-glucose (UDPG) as the sole glucose donor. An affective enzymatic system for repeated production of the monoglucoside has been developed by using an enzyme fraction as a water-insoluble biocatalyst in an enzyme membrane reactor (slurry reactor) equipped with an ultrafiltration membrane. The GTF also catalyzed the regioselective glucosylation of salicyl alcohol to salicin and of various polyphenols to naturally occurring pigments such as sugar-containing flavonoids. This study is the first to observe GTF activity in the enzyme fractions from the cultured cells of E. perriniana, which specifically catalyze the UDPG-dependent monoglucosylation of aromatic compopunds and flavonoids.
- 公益社団法人日本生物工学会の論文
- 1997-11-25
著者
-
中島 伸佳
Graduate School Of Health And Welfare Okayama Prefectural University
-
中島 伸佳
岡山県立大学短期大学部
-
中島 伸佳
岡山県立短大
-
ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
-
HAMADA Hiroki
Department of Applied Science, Okayama University of Science
-
FURUYA Tsutomu
Department of Applied Science, Okayama University of Science
-
MATSUMURA Shingo
Department of Applied Science, Okayama University of Science
-
Hamada H
National Institute Of Fitness And Sports In Kanoya
-
Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
-
Nakajima Nobuyoshi
Graduate School Of Health And Welfare Okayama Prefectural University
-
Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
-
Furuya Tsutomu
School Of Pharmaceutical Sciences Kitasato University
-
Furuya Tsutomu
Department Of Applied Sci. Okayama University Of Science
-
Matsumura Shingo
Department Of Applied Science Okayama University Of Science
-
FURUTYA TSUTOMU
Department of Applied Science, Okayama University of Science
-
MATSUMURA SHINGO
Nippon Olive Co. Ltd.,
-
MIZOBATA SATOSHI
Omnitech Corporation
-
Hamada Hiroki
Department Of Applied Sci. Okayama University Of Science
-
Furuya Tsutomu
Department of Applied Sci. , Okayama University of Science
-
Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
-
HAMADA Hiroki
Department of Applied Sci. , Okayama University of Science
関連論文
- イソクエルシトリンによるアコヤガイ赤変病の軽減
- 植物性素材を原料とした「乳酸菌・酵母発酵エキス(PELY)」に関する研究
- エコシステムを利用した瀬戸内海沿岸の海洋環境汚染物質の除去・回収法の開発--環境ホルモン関連物質を中心として
- 瀬戸内海総合開発特別研究(第36回伊原木記念賞)エコシステムを利用した瀬戸内海沿岸の海洋汚染物質の除去・回収法の開発
- 海洋性微細藻類を用いる内分泌攪乱物質(環境ホルモン)の機能改変と海洋汚染物質の除去に関する研究
- 食品添加物としての、天然植物色素の酵素的安定化と、抗酸化性を中心としたそれらの付随的生理機能の解明
- P-12 植物培養細胞による活性フェノール化合物の配糖化(ポスター発表の部)
- Further Characterization of Earthworm Serine Proteases : Cleavage Specificity Against Peptide Substrates and on Autolysis
- Multi-enzymatic Glucosylation Using Eucalyptus UDP-Glucosyltransferase Coupled UDPglucose-Fermentation by Bakers'Yeast
- Lipase-catalyzed Synthesis of Arbutin Cinnamate in an Organic Solvent and Application of Transesterification to Stabilize Plant Pigments
- Preparation of Optically Active α-Hydroxy Esters : Stereoselective Reduction of α-Keto Esters Using Thermophilic Actinomycetes
- Amino Acid Sequence and Characterization of Aldo-keto Reductase from Bakers' Yeast
- Stereoselective Reduction of α- and β-Keto Esters with Aerobic Thermophiles, Bacillus Strains
- Protein Sequences of Two Keto Ester Reductases: Possible Identity as Hypothetical Proteins
- Enzymatic Production of (S)-1-Acetoxy-2-Alkanol with Bakers' Yeast Cell-Free Extract in a Membrane Reactor
- 657 酵素共役反応系を用いるラセミ型アミノ酸から光学活性L-異性体への酵素的変換
- 2-I-23 NADH水素転移の立体化学 : NMRによるin situ解析法(第41回大会一般研究発表)
- 遺伝子組換え技術によるグルタミン酸ラセマ-ゼの酵素化学研究と,有用物質の酵素的生産
- ピリドキサルリン酸非依存性アミノ酸ラセマ-ゼ (1988年の化学-3-)
- A-8 乳酸菌のグルタミン酸ラセマーゼの反応機構(日本農芸化学会関西支部大会報告)
- 11.Bacillus stearothermophilusの新酵素,NAD要求性メゾジアミノピメリン酸脱水素酵素(第291回会議研究発表要旨,ビタミンB研究委員会)
- Multi-enzymatic Glucosylation Using Eucalyptus UDP-Glucosyltransferase Coupled UDPglucose-Fermentation by Bakers'Yeast
- Enzymatic Production of (S)-1-Acetoxy-2-Alkanol with Bakers' Yeast Cell-Free Extract in a Membrane Reactor
- A Novel Zinc-containing α-Keto Ester Reductase from Actinomycete : An Approach Based on Protein Chemistry and Bioinformatics
- 乳酸菌の生産する新規な2-ケト酸リダクタ-ゼ--精製と性質
- 黄色ブドウ球菌におけるタウリン輸送系の研究--細胞膜小胞における特性との比較
- Biotransformation of Cinnamic Acid, p-Coumaric Acid, Caffeic Acid, and Ferulic Acid by Plant Cell Cultures of Eucalyptus perriniana
- P-337 Synthesis and Activity of Acyl-substituted Catechin, Epicatechin and Procyanidin dimers
- Degradation of Pectic Substances by Two Pectate Lyases from a Human Intestinal Bacterium, Clostridium butyricum-beijerinckii Group
- Role of Hydrogen in Polycrystalline Si by Excimer Laser Annealing
- 成長に伴う鶏肝ヘキソナ-ゼおよび解糖系の変動
- Purification and Characterization of α-Keto Ester Reductases from Bakers' Yeast
- Differences in Protein Structure and Similarities in Catalytic Function of Two L-Stereoselective Carbonyl Reductases from Bakers' Yeast
- Enzymatic preparation of arbutin derivatives : Lipase-catalyzed direct acylation without the need of vinyl ester as an acyl donor(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Synthesis of Unnatural Mono- and Oligosaccharides of Farnesol, Geraniol, and (S)-Perillyl Alcohol by Biocatalytic Glycosylations
- Enhancement of Water-Solubility and Bioactivity of Paclitaxel Using Modified Cyclodextrins(METHODS)
- 有機溶媒に耐性で常温においては自己消化による失活を伴わない「ミミズプロテアーゼ」の物質合成・変換への応用 (特集 物質合成の設計図にブレークスルーを--酵素触媒の活躍と未来)
- Analysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone-Enriched Tempeh(BREWING AND FOOD TECHNOLOGY)
- An Isozyme of Earthworm Serine Proteases Acts on Hydrolysis of Triacylglycerol
- Biotransformation of (+)-catechin by plant cultured cells of Eucalyptus perriniana
- Purification and Characterization of α-Keto Amide Reductase from Saccharomyces cerevisiae
- Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp. PCC 7942
- Damage to Cultivated Japanese Pearl Oysters by Oxidative Stress That Was Related to "Mass Mortality"(Biochemistry & Molecular Biology)
- One- step Glucosylation of Capsaicinoids by Cultured Cells of Phytolacca americana
- Comparison of Acylated Plant Pigments : Light-resistance and Radical-scavenging Ability(Biochemistry & Molecular Biology)
- Further Stabilization of Earthworm Serine Protease by Chemical Modification and Immobilization(Biochemistry & Molecular Biology)
- Enzyme-catalyzed Acylation of Plant Polyphenols for Interpretation of Their Functions
- Stereoselective Reduction of Ketone and Enone using Plant Cell Cultures
- Purification and Characterization of Two α-Keto Ester Reductases from Streptomyces thermocyaneoviolaceus IFO 14271(Microbiology & Fermentation Technology)
- Functional Glucosylation of Kojic Acid and Daidzein with the Eucalyptus Membrane-Associated UDP-Glucosyltransferase Reaction System
- Stereocontrolled Reduction of α-and β-Keto Esters with Micro Green Algae, Chlorella Strains
- Regioselective Acylation of Flavonoid Glucoside with Aromatic Acid by an Enzymatic Reaction System from Cultured Cells of Ipomoea batatas
- カツオ(Katsuwonus pelamis)塩辛由来の新規な線溶プロテア-ゼ--精製と性質
- ミミズ(Lumbricus rubellus)細胞中に発見されたきわめて安定な線溶酵素-3-蛋白化学的性質と分子構造
- ミミズ(Lumbricus rubellus)細胞中に発見されたきわめて安定な線溶酵素-2-単離・精製と酵素科学的性質
- ミミズ(Lumbricus rubellus)細胞中に発見されたきわめて安定な線溶酵素-1-存在と性質,安定性
- 生体触媒の応用--酵素バイオリアクタ-を用いる各種の光学活性アミノ酸の新規な生物的合成・変換法の開発
- Grain Morphology of Recrystallized Polycrystalline-Si Film by Excimer Laser Annealing
- Synthesis and Activity of Pyrimidinylpropenamide Antibiotics : The Alkyl Analogues of Sparsomycin(Organic Chemistry)
- Indocarbazostatin and Indocarbazostatin B, Novel Inhibitors of NGF-induced Neuronal Differentiation in PC12 Cells : II. Isolation, Physicochemical Properties and Structural Elucidation
- Synthesis of Oligosaccharides of Genistein and Quercetin as Potential Anti-inflammatory Agents
- Biological Degradation of Taxol by Action of Cultured Cells on 7-Acetyltaxol-2"-yl Glucoside
- Glycosylation and Malonylation of Quercetin, Epicatechin, and Catechin by Cultured Plant Cells
- Synthesis of α-Tocopheryl Disaccharides as Potential Antiallergic Agents
- Biotransformation of monofluorophenols by cultured cells of Eucalyptus perriniana
- Regioselective hydroxylation, reduction, and glycosylation of diphenyl compounds by cultured plant cells of Eucalyptus perriniana
- Glycosylation of Vitamin E Homologue by Cultured Plant Cells
- Asymmetric Transformation of Enones with Synechococcus sp. PCC 7942
- A novel hydroxylase from Catharanthus roseus participating in the hydroxylation of 2-hydroxybenzoic acid
- Purification and Characterization of Konjac Glucomannan Degrading Enzyme from Anaerobic Human Intestinal Bacterium, Clostridium butyricum-Clostridium beijerinckii Group
- Chemical Modification of Earthworm Fibrinolytic Enzyme with Human Serum Albumin Fragment and Characterization of the Protease as a Therapeutic Enzyme
- Synthesis of β-Maltooligosaccharides of α-Tocopherol Derivatives by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activity
- Phenylpropanoids as a Protectant of Aluminum Toxicity in Cultured Tobacco Cells
- PD3 BIOSYNTHESIS OF 5-DEOXYFLAVONOIDS : 6'-DEOXYCHALCONE SYNTHASE ACTIVITY IN CULTURED LICORICE AND SOYBEAN CELLS
- Stable Earthworm Serine Proteases : Application of the Protease Function and Usefulness of the Earthworm Autolysate
- Biotransformation of Paclitaxel by Cell Suspension Cultures of Marchantia polymorpha
- Stereoselective Reduction of Carbonyl Compounds with Actinomycete: Purification and Characterization of Three α-Keto Ester Reductases from Streptomyces avermitilis
- Effects of cytokinins on the auxin requirement and auxin content of tobacco calluses
- Effects of Capsinoid on Serum and Liver Lipids in Hyperlipidemic Rats
- Effects of Additives in the Reduction Using Bakers' Yeast Cell-Free Extract
- Purification and Characterization of Aldehyde Reductase from Leuconostoc dextranicum
- Continuous Conversion to Optically Pure L-Methionine from D-Enantiomer Contaminated Preparations by an Immobilized Enzyme Membrane Reactor
- FACTORS AFFECTING VITAMIN E PRODUCTION USING PLANT CELL CULTURE OF CARTHAMUS TINCTORIUS
- Synthetic Studies of Proanthocyanidins. Highly Stereoselective Synthesis of the Catechin Dimer, Procyanidin-B3(Organic Chemistry)
- Hypolipidemic Effects of Ethanol Extracts of Citrus depressa and Annona atemoya, Typical Plant Foodstuffs in Okinawa, Japan on KKAy Mice Fed with Moderately High Fat Diet
- A Sensitive Radial Diffusion Method for the Determination of Several Proteases
- 機能性食品としての海洋生物--無脊椎動物および藻類中に見出された血栓溶解関連物質
- Biotransformation of Cinnamic Acid, p-Coumaric Acid, Caffeic Acid, and Ferulic Acid by Plant Cell Cultures of Eucalyptus perriniana
- Stereoselective Reduction of Carbonyl Compounds with Actinomycete : Purification and Characterization of Three α-Keto Ester Reductases from Streptomyces avermitilis
- 血栓溶解酵素ナットウキナ-ゼの活性測定法
- Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus
- Enzymatic Production of β-Thujaplicin 2-O-β-D-Glucoside in a Membrane Reactor Using an Insoluble UDP-Glucosyltransferase Fraction from Cultured Cells of Eucalyptus perriniana
- Lipase-Catalyzed Direct and Regioselective Acylation of Flavonoid Glucoside for Mechanistic Investigation of Stable Plant Pigments
- Stereoselective Reduction in Biotransformation of Androstane Derivatives by Cell Suspension Cultures of Marchantia Polymorpha
- Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm, Lumbricus rubellus, as a Novel Source of Biocatalyst
- Potent Fibrinolytic Enzyme from the Lysate of Katsuwonus pelamis Digestive Tract (Shiokara) : Purification and Characterization
- Molecular Cloning, Sequencing, and Expression of cDNA Encoding Serine Protease with Fibrinolytic Activity from Earthworm(Biochemistry & Molecular Biology)
- 海洋生物と血栓溶解関連物質
- Fibrinolytic Enzymes in Experimental Gout Induced by Urate Crystal(MSU)
- Glycosylation of trans-Resveratrol by Plant-Cultured Cells