A Novel Zinc-containing α-Keto Ester Reductase from Actinomycete : An Approach Based on Protein Chemistry and Bioinformatics
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概要
- 論文の詳細を見る
We have achieved the purification of an α-keto ester reductase (SCKER) from S. coelicolor A3(2) whole cells. SCKER proved to be a homotetramer of 132 kDa containing one equivalent of zinc ion per subunit. The enzyme differed from other α-keto ester reductases from microorganisms with regard to subunit structure and metal ion dependency. From a computer search using the protein data banks, the N-terminal amino acid sequence of SCKER was consistent with that of a possible zinc containing alcohol dehydrogenase in S. coelicolor A3(2). None of three hypothetical proteins of S. coelocor A3(2) having a high homology sequence with those of already purified α-keto ester reductases from S. thermocyaneoviolaceus [Yamaguchi, H., et al., Biosci. Biotechnol. Biochem., 66, 588–597 (2002)] was identical with that of SCKER.
- 社団法人 日本農芸化学会の論文
- 2004-10-23
著者
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中島 伸佳
Graduate School Of Health And Welfare Okayama Prefectural University
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中島 伸佳
岡山県立大学短期大学部
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中島 伸佳
岡山県立短大
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ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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Esaki Nobuyoshi
Institute For Chemical Research Kyoto University
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YAMAGUCHI Hitomi
Research and Development Center, Nagase & Co., Ltd.
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OMORI Taketo
Institute for Chemical Research, Kyoto University
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UEMURA Tadashi
Institute for Chemical Research, Kyoto University
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NAKAJIMA Nobuyoshi
Graduate School of Health and Welfare Science, Okayama Prefectural University
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Omori Taketo
Institute For Chemical Research Kyoto University
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Uemura Tadashi
Institute For Chemical Research Kyoto University
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Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
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Nakajima Nobuyoshi
Graduate School Of Health And Welfare Okayama Prefectural University
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Nakajima Noriyuki
Biotechno-logy Research Center Toyama Prefectural University
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Yamaguchi H
Research And Development Center Nagase & Co. Ltd.
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