Gene Cloning, Purification, and Characterization of Two Cyanobacterial NifS Homologs Driving Iron-Sulfur Cluster Formation
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概要
- 論文の詳細を見る
Iron-sulfur proteins are essential in the photosynthetic system and many other biological processes. We have isolated and characterized enzymes driving the formation of iron-sulfur clusters from Synechocystis sp. PCC6803. Two genes (slr0387 and sll0704), showing similarity to nifS of Azotobacter vinelandii, were cloned, and their gene products (SsCsd1 and SsCsd2) were purified. They catalyzed the desulfuration of L-cysteine. Reconstitution of a [2Fe-2S] cluster of cyanobacterial ferredoxin proceeded much faster in the presence of L-cysteine and either of these enzymes than when using sodium sulfide. These results suggest that SsCsd1 and SsCsd2 facilitate the iron-sulfur from L-cysteine. Synechocystis sp. PCC6803 has no gene coding for a protein with similarity to the N-terminal domain of NifU of A. vinelandii, which is believed to cooperate with NifS to assemble iron-sulfur clusters. Thus, the cluster formation in the cyanobacterium probably proceeds through a mechanism that is different from that in A. vinelandii.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
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Kurihara T
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Kurihara Tatsuo
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Esaki N
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Kyoto Univ.
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Esaki Nobuyoshi
Nstitute For Chemical Research Kyoto University
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Esaki Nobuyoshi
京都大学化学研究所 分子微生物科学
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Ezaki Nobuyoshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Kurihara Tatsuo
Institute for Chemical Research, Kyoto University
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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吉村 徹
Institute For Chemical Research Kyoto University
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YOSHIMURA Tohru
Institute for Chemical Research, Kyoto University
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MIHARA Hisaaki
Institute for Chemical Research, Kyoto University
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KATO Shinichiro
Institute for Chemical Research, Kyoto University
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Yoshimura T
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Inst. For Chemical Res. Kyoto Univ.
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Kurihara Tatsuo
Inst. For Chemical Res. Kyoto Univ.
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Mizuno Haruo
Departments Of Pediatrics And Neonatology Nagoya City University Graduate School Of Medical Sciences
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Mizutani H
Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.
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KATO Shin-ichiro
Institute for Chemical Research, Kyoto University
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Esaki N
Kyoto Univ.
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Kurihara T
Kyoto Univ. Kyoto
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Yoshimura Tohru
(present Adress)institute For Chemical Research. Kyoto University
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Yoshimura Tohru
Institute For Chemical Research Kyoto University:(present Address)department Of Applied Molecular Bi
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Kato Shinichiro
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Mizutani Hisashi
Department Of Chemistry Faculty Of Science Osaka City University
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Murai Hiroki
名古屋市立大学 医学研究科新生児･小児医学分野
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Kurihara Tatsuo
Institute For Chemical Research Kyoto University
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Mihara Hisaaki
Institute For Chemical Research Kyoto University
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