Thermolabile Alanine Racemase from a Psychrotroph, Pseudomonas fluorescens : Purification and Properties

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概要

• 論文の詳細を見る

• A psychrotrophic bacterium that produces a thermolabile alanine racemase was isolated from raw milk, and identified as Pseudomonas fluorescens TM5-2. The enzyme was purified to homogeneity from the cell extract, and characterized to be compared with enzymes from mesophiles (Bacillus subtilis and Salmonella typhimurium) and a thermophile (Bacillus stearothermophilus). The enzyme has a molecular weight of about 76,000 and consists of two subunits identical in molecular weight (38,000). The enzyme contains two mol of pyridoxal 5'-phosphate per mol as a coenzyme. The amino acid composition was different from those of other alanine racemases in content of valine. The amino acid sequence of the amino terminal region (from ^1Met to ^<25>Gly) had 21-33% homology with those of other alanine racemases. Kinetic parameters of the enzyme were similar to those of other alanine racemases. The enzyme is extremely labile over 30℃, and shows the high catalytic activity even at 0℃; it is thermolabile and psychrotrophic.

• 社団法人日本農芸化学会の論文
• 1993-01-23

著者

• Esaki Nobuyoshi

  Kyoto Univ.

• Esaki Nobuyoshi

  Nstitute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• SODA Kenji

  Institute for Chemical Research, Kyoto University

• Soda Kenji

  Department Of Biotechnology Kansai University

• Soda Kenji

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• YOKOIGAWA Kumio

  Department of Food Science and Nutrition, Nara Women's University

• KAWAI Hiroyasu

  Department of Food Science and Nutrition, Nara Women's University

• Lim Young

  Institute For Chemical Research Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• ENDO Keiji

  Institute for Chemical Research, Kyoto University

• Soda K

  Department Of Biotechnology Kansai University

• Kawai Hiroyasu

  Department Of Food Science And Nutrition Nara Women's University

• Endo Kinji

  Department of Food Science and Nutrition, Nara Women's University

• Endo Kinji

  Department Of Food Science And Nutrition Nara Women's University

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Endo K

  Rengo Co. Ltd. Osaka Jpn

• Yokoigawa K

  Nara Women's Univ. Nara Jpn

• Yokoigawa Kumio

  Department Of Food Science And Nutrition Nara Women's University

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