The Role of Cysteine 116 in the Active Site of the Antitumor Enzyme L-Methionine γ-Lyase from Pseudomonas putida

スポンサーリンク

概要

• 論文の詳細を見る

• The cysteinyl residue at the active site of L-methionine γ-lyase from Pseudomonas putida (MGL_Pp) is highly conserved among the heterologous MGLs. To determine the role of Cys116, we constructed 19 variants of C116X MGL_Pp by saturation mutagenesis. The Cys116 mutants possessed little catalytic activity, while their affinity for each substrate was almost the same as that of the wild type. Especially, the C116S, C116A, and C116H variants composed active site catalytic function as measured by the kinetic parameter kcat toward L-methionine. Furthermore, the mutagenesis of Cys116 also affected the substrate specificity of MGL_Pp at the active center. Substitution of Cys116 for His led to a marked increase in activity toward L-cysteine and a decrease in that toward L-methionine. Propargylglycine inactivated the WT MGL, C116S, and C116A mutants. Based on these results, we postulate that Cys116 plays an important role in the γ-elimination reaction of L-methionine and in substrate recognition in the MGLs.

• 社団法人 日本農芸化学会の論文

著者

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• TAMURA Takashi

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Yamamoto Masaki

  Graduate School Of Natural Science And Technology Okayama University

• KUDOU Daizou

  Graduate School of Natural Science and Technology, Okayama University

• MISAKI Shintaro

  Shionogi and Co., Ltd.

• YAMASHITA Masao

  Graduate School of Natural Science and Technology, Okayama University

• INAGAKI Kenji

  Graduate School of Natural Science and Technology, Okayama University

関連論文

• Production of (S)-2-Chloropropionate by Asymmetric Reduction of 2-Chloroacrylate with 2-Haloacrylate Reductase Coupled with Glucose Dehydrogenase(BIOCHEMICAL ENGINEERING)
• Serine Racemase with Catalytically Active Lysinoalanyl Residue
• Gene Cloning, Purification, and Characterization of 2, 3-Diaminopropionate Ammonia-lyase from Escherichia coli(Biochemistry & Molecular Biology)
• Gene Cloning, Purification, and Characterization of Two Cyanobacterial NifS Homologs Driving Iron-Sulfur Cluster Formation
• Stereochemistry of the Transamination Reaction Catalyzed by Aminodeoxychorismate Lyase from Escherichia coli: Close Relationship between Fold Type and Stereochemistry
• Three-Dimensional Structure of 4-Amino-4-Deoxychorismate Lyase from Escherichia coli
• Kinetic and Mutational Studies of Three NifS Homologs from Esherichia coli: Mechanistic Difference between L-Cysteine Desulfurase and L-Selenocysteine Lyase Reactions
• A Novel Zinc-containing α-Keto Ester Reductase from Actinomycete : An Approach Based on Protein Chemistry and Bioinformatics
• Mechanism of Inactivation of α-Amino-ε-caprolactam Racemase by α-Amino-δ -valerolactam
• Synthesis of Selenocystine and Selenohomocystine with O-Acetylhomoserine Sulfhydrylase
• Cloning and Expression of the Pseudomonas Gene for Utilization of D-Leucine in Escherichia coli
• Action of S-Carbamoyl and S-Thiocarbamoyl Derivatives of L-Cysteine on L-Amino Acid Oxidase
• Inactivation of Amino Acid Racemase by S-(N-Methylthiocarbamoyl)- D, L-cysteine
• Reactions of O-Substituted L-Homoserines Catalyzed by L-Methionine y-Lyase and Their Mechanism
• Purification and Properties of L-Methionine γ-Lyase from Aeromonas sp.
• Enzymatic Synthesis of S-Substituted L-Cysteines with Tryptophan Synthase of Escherichia coli
• Properties of α-Amino-ε-caprolactam Racemasefrom Achromobacter obae
• X-Ray Structure of a Reaction Intermediate of L-2-Haloacid Dehalogenase with L-2-Chloropropionamid
• Production of New Tricarboxylic Acid Anhydrides from Stearic Acid by Pseudomonas cepacia A-1419
• Biotransformation of Oleic Acid by Alcaligenes sp. 5-18,a Bacterium Tolerant to High Concentrations of Oleic Acid
• Transformation of Oleic Acid and Its Esters by Sarcina lutea(Microbiology & Fermentation Industry)
• Identification of Proteins Interacting with Selenocysteine Lyase
• Enzymatic Synthesis of L-Pipecolic Acid by Δ^1-Piperideine-2-carboxylate Reductase from Pseudomonas putida
• Selenoprotein Biosynthesis and Selenium-Specific Enzymes (特集1:若手が拓く微量元素研究の最前線)
• Protein Interaction between Selenophosphate Synthetase and IscS
• RNAi-mediated knock down of mammalian selenocysteine lyase
• A New Family of NAD(P)H-Dependent Oxidoreductases Distinct from Conventional Rossmann-Fold Proteins
• Network of Protein-Protein Interactions among Iron-Sulfur Cluster Assembly Proteins in Escherichia coli
• Structure of External Aldimine of Escherichia coli CsdB, an IscS/NifS Homolog: Implications for Its Specificity toward Selenocysteine
• Tyrosine 265 of Alanine Racemase Serves as a Base Abstracting α-Hydrogen from L-Alanine: The Counterpart Residue to Lysine 39 Specific to D-Alanine
• Host movement initiates oviposition behavior of Meteorus pulchricornis, a braconid parasitoid of free-living lepidopteran larvae
• Crystal Structure of H_2-Proteinase from the Venom of Trimeresurus flavoviridis
• Crystallization and Preliminary X-Ray Study of H_2-Proteinase from the Venom of Trimeresurus flavoviridis
• Role of Tyrosine 265 of Alanine Racemase from Bacillus stearothermophilus
• The Distribution of Phosphatidyl-D-serine in the Rat
• Primary Structure and Properties of Bacterial Formate Dehydrogenase From Moraxella sp C-1
• Thermostable Ornithine Aminotransferase from Bacillus sp. YM- 2: Purification and Characterization
• Structural Analysis of the L-Methionine γ-Lyase Gene from Pseudomonas putida^1
• In Vivo Effect of GroESL on the Folding of Glutamate Racemase of Escherichia coli
• Large-Scale Production of Thermostable Alanine Dehydrogenase from Recombinant Cells
• 411 Enzymatic Dehalogenation of 2-Halo Acids in Nonaqueous Medium
• Characterization of Human Selenocysteine Synthase Involved in Selenoprotein Biosynthesis
• Functional Analysis of Two Homologous Mouse Selenophosphate Synthetases
• DnaK from Vibrio proteolyticus : Complementation of a dnaK-Null Mutant of Escherichia coli and the Role of Its ATPase Domain (Enzymology, Protein Engineering, and Enzyme Technology)
• Higher Yielding Isolation of Kinsenoside in Anoectochilus and Its Antihyperliposis Effect
• Kinetics of Thermostable Alanine Racemase of Bacillus stearothermophilus
• Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene
• Crystal Structure of the Pyridoxal 5'-phosphate Dependent L-Methionine γ-Lyase from Pseudomonas putida
• Structure of the Antitumour Enzyme L-Methionine γ-Lyase from Pseudomonas putida at 1.8A Resolution
• Role of Tyrosine 114 of L-Methionine γ-lyase from Pseudomonas putida
• Transamination as a Side-Reaction catalyzed by Alanine Racemase of Bacillus stearothermophilus
• Construction and Properties of a Fragmentary D-Amino Acid Aminotransferase
• Stereospecific Labeling at α-Position of Phenylalanine and Phenylglycine with Amino Acid Racemase
• Compensation for D-Glutamate Auxotrophy of Escherichia coli WM335 by D-Amino Acid Aminotransferase Gene and Regulation of murI Expression
• Mutation of Arginine 98, Which Serves as a Substrate-Recognition Site of D-Amino Acid Aminotransferase, Can Be Partly Compensated for by Mutation of Tyrosine 88 to an Arginyl Residue
• Cloning and Expression of the Glutamate Racemase Gene of Bacillus pumilus^1
• Conversion of α-Keto Acids to D-Amino Acids by Coupling of Four Enzyme Reactions
• Glutamate Racemase of E. coli : Recharacterization of the Activation by UDP-N -Acetylmuramoyl-L-Alanine
• Reconstitution of Fragmentary Form of Thermostable Alanine Racemase
• A Novel NADH-Dependent Carbonyl Reductase from Kluyveromyces aestuarii and Comparison of NADH-Regeneration System for the Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutanoate
• Catalytic Action of L-Methionine γ-Lyase on 4-Azaleucine(Biological Chemistry)
• Expression of a spinach gene encoding a cysteine synthase-like protein in tobacco plants
• Chemical Modification of Cysteine Residues of _L-Methionine γ-Lyase(Biological Chemistry)
• Effects of Pyridoxal 5'-Phosphate on the Refolding of Aspartate Aminotransferase
• Inactivation of Glutamate Racemase of Pediococcus pentosaceus with L-Serine O-Sulfate
• Thermolabile Alanine Racemase from a Psychrotroph, Pseudomonas fluorescens : Purification and Properties
• Total Conversion of Racemic Pipecolic Acid into the L-Enantiomer by a Combination of Enantiospecific Oxidation with D-Amino Acid Oxidase and Reduction with Sodium Borohydride
• Synthesis of L-Proline form the Racemate by Coupling of Enzymatic Enantiospecific Oxidation and Chemical Non-Enantiospecific Reduction
• Decomposition of L-Selenodjenkolate Catalyzed by S-Alkylcysteine α,β-Lyase(Biological Chemistry)
• Thermostable S-Alkylcysteine α,β-Lyase from a Thermophile : Purification and Properties(Biological Chemistry)
• Effect of Tilted Edges on Coupled Vibrations of Doubly Rotated Quartz Plates
• Gene Cloning, Purification and Characterization of Thermostable Alanine Dehydrogenase of Bacillus stearothermophilus
• Prospects for X-ray Crystal Structure Analysis of Selenoproteins with SPring-8 Synchrotron Radiation
• The Role of Cysteine 116 in the Active Site of the Antitumor Enzyme L-Methionine γ-Lyase from Pseudomonas putida
• The Role of Cysteine 116 in the Active Site of the Antitumor Enzyme L-Methionine γ-Lyase from Pseudomonas putida
• The Distribution of Phosphatidyl-D-serine in the Rat
• Selenocysteine Is Selectively Taken Up by Red Blood Cells
• Identification of Proteins Interacting with Selenocysteine Lyase
• Expression analysis of mammalian selenocysteine lyase
• Determination by ^1H-NMR of the Stereospecificity of NAD-dependent Plant L-Histidinol Dehydrogenase for Nicotinamide C-4 Hydrogen Transfer
• Protein Interaction between Selenocysteine Lyase and MUP-I
• Mammalian Selenocysteine Lyase Is Involved in Selenoprotein Biosynthesis
• The Role of Amino Acid Residues in the Active Site of L-Methionine γ-lyase from Pseudomonas putida
• The Role of Amino Acid Residues in the Active Site of L-Methionine γ-lyase from Pseudomonas putida

もっと見る 閉じる

スポンサーリンク