Effects of Pyridoxal 5'-Phosphate on the Refolding of Aspartate Aminotransferase

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概要

• 論文の詳細を見る

• The effects of pyridoxal 5'-phosphate on the refolding of aspartate aminotransferase were studied. The refolding of the cytoplasmic isozyme of pig heart aspartate aminotransferase denatured with 6 M guanidine hydrochloride depends critically on the presence of pyridoxal 5'-phosphate. Pyridoxal 5'-phosphate affects a refolding intermediate at guanidine hydrochloride concentrations between 3.5 and 2.0 M to bring out the correct folding. In contrast, thermostable aspartate aminotransferase of Bacillus sp. YM-2 can be refolded independently of pyridoxal 5'-phosphate.

• 社団法人日本農芸化学会の論文
• 1994-02-23

著者

• Esaki N

  Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Kyoto Univ.

• Esaki Nobuyoshi

  Nstitute For Chemical Research Kyoto University

• Ezaki Nobuyoshi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• YOSHIMURA Tohru

  Institute for Chemical Research, Kyoto University

• SODA Kenji

  Institute for Chemical Research, Kyoto University

• Soda Kenji

  Department Of Biotechnology Kansai University

• Soda Kenji

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• Yoshimura Tohru

  Institute For Chemical Research Kyoto University:(present Address)department Of Applied Molecular Bi

• Esaki Nobuyoshi

  Kyoto Univ. Kyoto Jpn

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Wakayama Mamoru

  Institute. For Chemical Research Kyoto University

• Soda K

  Department Of Biotechnology Kansai University

• Kim Woon

  Institute. for Chemical Research, Kyoto University

• Wakayama Mamoru

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Kim Woon

  Institute. For Chemical Research Kyoto University

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