Two Kinds of 2-Halo Acid Dehalogenases from Pseudomonas sp. YL Induced by 2-Chloroacrylate and 2-Chloropropionate

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概要

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• The bacterial isolates WS, WL, and YL, which were identified as Pseudomonas, grew in 2-chloroacrylate (2-CAA) and 2-chloropropionate (2-CPA) media to produce 2-halo acid dehalogenases. Pseudomonas sp. YL showed the highest 2-CPA dehalogenase activity among the three strains, and inducibly produced two kinds of 2-halo acid dehalogenases in a medium containing either 2-CAA or 2-CPA. The 2-CAA-induced enzyme catalyzed dehalogenation of D- and L-2-CPA to produce L- and D-lactate, respectively. Its pH optimum of the dehalogenation and activity staining indicated that a single enzyme catalyzes the dehalogenation of both enantiomers of 2-CPA. The 2-CPA-induced enzyme catalyzed production of D-lactate from L-2-CPA, but did not act on D-2-CPA. The two dehalogenases are different from each other in mobility upon polyacrylamide gel electrophoresis and sensitivity towards thiol reagents.

• 社団法人日本農芸化学会の論文
• 1994-09-23

著者

• Kurihara T

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Kurihara T

  Kyoto Univ. Kyoto Jpn

• Kurihara Tatsuo

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Esaki N

  Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Kyoto Univ.

• Esaki Nobuyoshi

  Nstitute For Chemical Research Kyoto University

• Ezaki Nobuyoshi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Inst. For Chemical Res. Kyoto Univ.

• SODA Kenji

  Laboratory of Microbiology, Institute for Chemical Research, Kyoto University

• Soda Kenji

  Department Of Biotechnology Kansai University

• Soda Kenji

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• Hasan A.k.m.

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• LIU Ji-Quan

  Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University

• Takada Harumi

  Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University

• Koshikawa Hiromoto

  Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University

• Liu Ji-quan

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Kurihara T

  Kyoto Univ. Kyoto

• Esaki Nobuyoshi

  Kyoto Univ. Kyoto Jpn

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Soda K

  Department Of Biotechnology Kansai University

• Takada Harumi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Koshikawa Hiromoto

  Department Of Environmental & Sanitary Engineering Faculty Of Engineering Kyoto University

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