Localization of Candida tropicalis Peroxisomal Isocitrate Lyase Highly Expressed in Peroxisome-Proliferated Saccharomyces cerevisiae

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概要

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• The proliferation of peroxisomes was observed in Saccharomyces cerevisiae MT8-1 cells grown in a medium containing 0.5% oleic acid and 0.1% glucose as the carbon sources, together with the induction of peroxisomal enzymes, such as catalase and acyl-CoA oxidase. The results of the subcellular fractionation and Western blot analysis indicated that Candida tropicalis peroxisomal isocitrate lyase (ICL) highly expressed in S. cerevisiae under the activation of the GAL7 promoter (Oda et al., Arch. Microbiol., 156,439-443,1991) was partially trasported in the particulate fraction containing peroxisomes and mitochondria. Furthermore, observation under immunocytochemical electronmicroscopy revealed that the enzyme protein was at least partially localized inside peroxisomes proliferated in S. cerevisiae, but not in mitochondria. These facts indicated that the peroxisomal targeting signal of C. tropicalis ICL seemed to be able to function in S. cerevisiae, but that the ICL expressed was not sufficiently accommodated into peroxisomes, probably due to the overproduction of the enzyme protein for S. cerevisiae peroxisomes.

• 社団法人日本生物工学会の論文
• 1992-12-25

著者

• Kurihara T

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Kurihara Tatsuo

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Kamasawa N

  日本女子大学理学部

• UEDA MITSUYOSHI

  Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist

• TANAKA ATSUO

  Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist

• NAITO NOBUKO

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• KAMASAWA NAOMI

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• OSUMI MASAKO

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• Tanaka A

  Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute

• Atomi H

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Atomi Haruyuki

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• KAMADA YASUSHI

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, K

• ODA KEINOSUKE

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, K

• UKITA RIE

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• Kurihara T

  Kyoto Univ. Kyoto

• Tanaka A

  京都大学大学院工学研究科

• UEDA Mitsuyoshi

  京都大学大学院工学研究科

• Osumi Masako

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Osumi Masako

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Woman's University

• Atomi Haruyuki

  Dept. Synth. Chem. Biol. Chem. Grad. Sch. Eng. Kyoto Univ.:crest Imanaka Project

• Tanaka A

  Mie Univ. Mie Jpn

• Tanaka A

  Kao Corp. Wakayama Jpn

• Ukita Rie

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Naito Nobuko

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Naito Nobuko

  Department Of Anatomy School Of Medicine Showa University

• Kamada Yasushi

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Takagi Atsuko

  Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute

• Oda Keinosuke

  Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot

• Kamasawa Naomi

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Tanaka Atsuo

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr

• Ueda Mitsuyoshi

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Ueda Mitsuyoshi

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Osumi Masako

  Department Of Biology Faculty Of Science Osaka City University

• Tanaka Atsuo

  Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot

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