Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin

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概要

• 論文の詳細を見る

• Phytochelatins (PCs, (γGlu-Cys)_n-Gly (n=2-11)) are produced by higher plants, algae, and some fungi in response to heavy metal ion exposure. A rapid and convenient method for quantifying heavy metal ion concentrations in water environments was developed using a chemically synthesized PC as a mediator. The chelating ability of the PC and quantification of the thiol group were utilized to measure heavy metal ions at low concentrations. The method requires only ten minutes for measurement and only 1 ml of a Iiquid sample. A range of homogeneous PCs (n=4-7) were chemically synthesized using a peptide synthesizer. These, especially PC_7, exhibited higher sensitivity and consistency of measurement than the native PC from Silene cucubalus, which produced a mixture of PC_2, PC_3, and PC_4. Detoxification of heavy metal ions in vitro by PC was also investigated. Using the paper disc method, the cell growth inhibition zone caused by cadmium ion against Salmonella typhimurium TA1538 was significantly decreased by addition of PC. Furthermore, at the minimum inhibitory concentration of cadmium ion (200 μM) in a nutrient broth culture of S.typhimurium, cell growth was almost completely recovered by addition of PC to the medium.

• 社団法人日本生物工学会の論文
• 1999-09-25

著者

• Takagi Masahiro

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Hirata Kazumasa

  Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University

• Takagi M

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• SATOFUKA Hiroyuki

  School of Materials Science, Japan Advanced Institute of Science and Technology

• AMANO Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• TAKAGI MASAHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Hashimoto K

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• HIRATA KAZUMASA

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• MIYAMOTO KAZUHISA

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• ATOMI HARUYUKI

  Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un

• Takagi M

  Nagasaki Univ. Nagasaki Jpn

• Miyaoku Kohei

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Atomi H

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Atomi Haruyuki

  Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr

• Atomi Haruyuki

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• SATOFUKA HIROYUKI

  Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un

• Hirata Kazumasa

  Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University

• Hirata Kazumasa

  Environmental Bioengineering Laboratory Graduated School Of Pharmaceutical Sciences Osaka University

• Hirata Kazumasa

  Environ. Bioeng. Lab Grad. Sch. Pharm. Sci. Osaka Univ.

• Miyamoto Kazuhisa

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Miyamoto Kazuhisa

  Environmental Bioengineering Laboratory Graduated School Of Pharmaceutical Sciences Osaka University

• Miyamoto Kazuhisa

  Laboratory Of Biological Defenoe Department Of Insect Physiology And Behavior National Institute Of

• Satofuka Hiroyuki

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Atomi Haruyuki

  Dept. Synth. Chem. Biol. Chem. Grad. Sch. Eng. Kyoto Univ.:crest Imanaka Project

• Miyamoto K

  Environmental Biotechnology Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Amano Satoshi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Hirata K

  Environmental Biotechnology Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Miyamoto K

  Faculty Of Pharmaceutical Sciences Osaka University

• Hirata Kazumasa

  Osaka Municipal Technical Research Institute

• Imanaka T

  Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.

• Miyamoto Kazuhisa

  Environmental Bioengineering Laboratory Faculty Of Pharmaceutical Sciences Osaka University

• Takagi Masahiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

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