Chelation of cadmium ions by phytochelatin synthas : role of the cystein-rich C-terminal
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概要
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The interactions between Cd^<2+> and the C-terminal region of phytochelatin (PC) synthase using recombinant wild-type and mutant PC synthase were studied. We show that site-directed mutagenesis of Cys residues at C^<358>C^<359>XXXC^<363>XXC^<366> motif decreases the number of Cd^<2+> and other heavy metal ions interacting with the enzyme, and that the motif binds the metals discriminatingly. The optimum binding ratio of PC synthase to Cd^<2+> was also determined. The findings indicate that Cys exists as a free SH residue and that it is involved in the regulation of PC enzyme activity by transferring the metals into closer proximity with the catalytic domain. These results are important in understanding heavy metal detoxification mechanisms in higher plants, a step towards phytoremediated-applications.
- 2008-02-10
著者
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Takagi Masahiro
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Takagi M
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Takagi Mutsumi
International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A
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Takagi M
Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn
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Hirata Kazumasa
Department of Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka Unive
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Takagi M
Nagasaki Univ. Nagasaki Jpn
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SHIRAKI Kentaro
Institute for Applied Physics, University of Tsukuba
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Shiraki K
Institute Of Applied Physics University Of Tsukuba
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Shiraki Kentaro
Institute For Applied Physics University Of Tsukuba
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MATSUMOTO Sachiko
School of Materials Science, Japan Advanced Institute of Science and Technology
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NISHIKORI Shingo
Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto Univers
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Hirata Kazumasa
Department Of Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osa
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Takagi Mutsumi
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Takagi Masahiro
School Of Material Science Japan Advanced Institute Of Science And Technology
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Vestergaard Mun'delanji
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Nishikori Shingo
Division Of Molecular Cell Biology Institute Of Molecular Embryology And Genetics Kumamoto Universit
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Takagi Masahiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Matsumoto Sachiko
School Of Materials Science Japan Advanced Institute Of Science And Technology
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