Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
スポンサーリンク
概要
- 論文の詳細を見る
The effects of antioxidants on the apoptosis of Chinese hamster ovary(CHO)1-15, 500 cells and production of tissue plasminogen activator(tPA)in suspension culture were investigated. After cell growth to 2×10^5 cells/ml in Ham's F12 medium containing 10% newborn bovine serum(NBS)in a spinner bottle, CHO cells were maintained for 6 d in Ham's F12 medium containing 0 or 0.4% NBS and 10 mM antioxidants, namely, _L-ascorbic acid 2-phosphate(VCP)or the reduced form of glutathione(GSH). The viable cell concentrations at day 6 in the serum-free culture with GSH and in the low-serum culture with VCP or GSH were 0.57, 1.04 and 1.69×10^5 cells/ml, respectively, while those in the serum-free and low-serum cultures without the antioxidants were only 2.33 and 1.17×10^3 cells/ml, respectively. The percentages of apoptotic cells in the serum-free and low-serum cultures with VCP(73.2, 44.6%)and GSH(76.9, 38.6%)measured using a flowcytometer after low-serum cultures with VCP(73.2, 44.6%)and GSH(76.9, 38.6%)measured using a flowcytometer after annexin V-FITC/propidium iodide staining were markedly lower than those in the cultures without antioxidants(96.3, 92.5%). The percentage of cells having a high mitochondrial membrane potential among the viable cells in the cultures with antioxidants determined using a flowcytometer after 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolylcarbocyanine iodide staining was clearly higher than those in the cultures without the antioxidants. The production of tPA in the serum-free and low-serum media with VCP(0.282, 2.02 mg/l)or GSH(1.01, 1.61 mg/l)was markedly higher than that in the cultures without the antioxidants(0.275, 0.689 mg/l). Consequently, the suppression of apoptosis through the maintenance of the membrane potential of mitochondria by VCP or GSH resulted in a marked increase in tPA production by CHO cells in the serum-free and low-serum cultures.
- 社団法人日本生物工学会の論文
- 2001-06-25
著者
-
TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
-
YOSHIDA Toshiomi
International Center for Biotechnology, Osaka University
-
Yoshida Toshiomi
大阪大学生物工学国際交流センター
-
Yoshida Toshiomi
International Center For Biotechnology Osaka University
-
吉田 豊和
岐阜大・工・生命工
-
Takagi Mutsumi
International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A
-
Takagi M
International Center For Biotechnology Osaka University
-
Takagi M
Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn
-
Takagi M
Osaka Univ. Osaka Jpn
-
Yoshida T
Kinki Univ. Nara Jpn
-
Takagi Mutsumi
Internatioal Center For Biotechnology Osaka University
-
Yun Zhanyou
International Center For Biotechnology Osaka University
-
Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
-
吉田 豊和
岐阜大 工
関連論文
- Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
- Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
- 611 2-ビドロキシ-4-メチルチオ酪酸アミド生産菌のニトリルヒドラターゼ遺伝子のクローニングと解析(酵素・酵素工学・タンパク質工学,一般講演)
- 923 NHaseを用いた2-ヒドロキシ-4-メチルチオ酪酸アミドの生産
- 2A16-4 epsilon-Poly-L-lysine(PL)分解酵素遺伝子の取得と破壊株を用いた機能解析(遺伝子工学・核酸工学,一般講演)
- Human N-Acetylglucosaminyltransferase I. : Expression in Escherichia coli as a Soluble Enzyme, and Application as an Immobilized Enzyme for the Chemoenzymatic Synthesis of N-Linked Oligosaccharides
- Construction of Xylose-Assimilating Saccharomyces cerevisiae
- Control of Cell Growth Rate by Sugar Feeding Based on CO_2 Production Rate
- Production of S-(+)-Ibuprofen with High Optical Purity from a Nitrile Compound by Cells immobilized on Cellulose Porous Beads
- Production of S-(+)-Ibuprofen from a Nitrile Compound by Enzymatic Reaction Combined with Ultrafiltration
- Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
- Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
- グリーンバイオプロセスの開発 : アクリルアミド工業化研究から学ぶ点
- 570 天然資源ロジンの微生物変換(酵素・酵素工学・タンパク質工学,一般講演)
- Divergent Structures of Carbazole Degradative car Operons Isolated from Gram-negative Bacteria
- Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively(Microbiology & Fermentation Technology)
- Expression, Purification, and Characterization of 2'-Aminobiphenyl-2,3-diol 1,2-dioxygenase from Carbazole-degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- Purification and Characterization of meta-Cleavage Compound Hydrolase from a Carbazole Degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- Organization and Transcriptional Characterization of Catechol Degradation Genes Involved in Carbazole Degradation by Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- New Classification System for Oxygenase Components Involved in Ring-Hydroxylating Oxygenations
- 3P-1072 エナンチオ選択的イミン還元酵素の探索と特徴解析(2a酵素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- Purification, Characterization and Gene Cloning of Thermostable Ο-Acetyl-L-Homoserine Sulfhydrylase Forming γ-Cyano-α-Aminobutyric Acid (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification, Characterization and Gene Cloning of Thermostable ο-Acetyl-L-Serine Sulfhydrylase Forming β-Cyano-L-Alanine
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
- Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
- Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- 2E09-3 炭酸固定反応を触媒する3,4-ジヒドロキシ安息香酸脱炭酸酵素の反応特性と一次構造解析(生物化学工学,一般講演)
- 3A10-1 炭酸固定反応を触媒する4-ヒドロキシ安息香酸脱炭酸酵素の反応特性と一次構造解析(酵素学・酵素工学・タンパク質工学,一般講演)
- 1A15-3 微生物触媒による 2,6-ジメチルピリジンのメチル基酸化反応
- 569 微生物触媒による芳香族カルボン酸のアルコールへの還元(酵素・酵素工学・タンパク質工学,一般講演)
- Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
- Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
- Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Effect of αl, 2-Mannosidic Linkage Located in α1, 3-Branch of Man6GlcNAc2 Oligosaccharide on Enzyme Activity of Recombinant Human Man_9-Mannosidase Produced in Escherichia coli
- Characterization of Recombinant Human Man_9-Mannosidase Expressed in Escherichia coli
- A Mass Spectrometry Membrane Probe and Practical Problems Associated with Its Application in Fermentation Processes
- On-Line Monitoring and Control of Aceotone-Butanol Fermentation by Membrane-Sensor Mass Spectrometry
- 1E14-4 Alcaligenes sp. IFO14130の菊酸エチルエステルと6-アミノヘキサン酸環状ダイマーに作用する加水分解酵素の関係(生物化学工学,一般講演)
- Application of the Fuzzy Theory to Simulation of Batch Fermentation
- 413 Simulation of Fermentation Process by Using Fuzzy Set Theory
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Metabolic Flux Change in Hybridoma Cells under High Osmotic Pressure
- 524 Improvement of Monoclonal Antibody Production with Gradual Increase of Osmotic Pressure
- Effect of Insoluble Medium Component on the Productivity of Tissue Plasminogen Activator by Cells on Microcarriers
- An Oxygen Supply Strategy for the Large-Scale Production of Tissue Plasminogen Activator by Microcarrier Cell Culture
- Global and Local Neural Network Models in Biotechnology : Application to Different Cultivation Processes
- 519 Control of Acetic Acid Accumulation during Cultivation of Recombinant E__-. coli AT2471.
- Characteristic of the Oligosaccharide Structure of Jack Bean α-Mannosidase with Some Lectins
- Production of Monascus Pigments by a Solid-Liquid State Culture Method
- Physiologically Motivated Strategies for Control of the Fed-Batch Cultivation of Recombinant Escherichia coli for Phenylalanine Production
- 921 炭酸固定反応をも触媒する脱炭酸酵素の探索 : インドール-3-カルボン酸脱炭酸酵素の発見
- 1E14-3 アミダーゼが触媒するアミドの生成反応(生物化学工学,一般講演)
- Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
- Synthesis and Evaluation of Novel Peptide-Lipid
- Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
- Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
- Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
- Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
- 1116 Dye-metal affinity chromatography in mixed mode for purification of alcohol dehydrogenase
- 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
- 1E13-4 2-ブチル-1,3-プロパンジオールの微生物交換(生物化学工学,一般講演)
- Novel Reversible Indole-3-carboxylate Decarboxylase Catalyzing Nonoxidative Decarboxylation(Microbiology & Fermentation Technology)
- Continuous Ethanol Production by a Concentrated Culture of Flocculating Yeast
- Control of Water Content in a Solid-State Culture of Aspergillus oryzae
- An Ingenious Device for Better Prediction of Bio-Processes Using a Multiple Regression Model : A case Study on Glucose Isomerase Production
- An Expert Approach for Control of Fermentation Processes as Variable Structure Plants
- Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Combined Addition of Glutathione and Iron Chelators for Decrease of Intracellular Level of Reactive Oxygen Species and Death of Chinese Hamster Ovary Cells
- Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
- Isolation of an Alginate-Degrading Organism and Purification of Its Alginate Lyase
- In Vitro Proliferation of Primary Rat Hepatocytes Expressing Ureogenesis Activity by Coculture with STO Cells
- Glucose Feeding Strategy Accounting for the Decreasing Oxidative Capacity of Recombinant Escherichia coli in Fed-Batch Cultivation for Phenylalanine Production
- Dewatering of Extracellular Polymer and Activated Sludges by Thermochemical Treatment
- Properties of Extracellular Polymer Having an Effect on Expression of Activated Sludge
- Dewatering Characteristics of Activated Sludges and Effect of Extracellular Polymer
- Computer-Aided On-Line Monitoring of Physiological Variables in Suspended Cell Cultures of Perilla frutescens in a Bioreactor
- Effects of High Concentrations of Energy Sources and Metabolites on Suspension Culture of Chinese Hamster Ovary Cells Producing Tissue Plasminogen Activator
- Effect of Mixing Time on Taxoid Production Using Suspension Cultures of Taxus chinensis in a Centrifugal Impeller Bioreactor
- Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line
- Starch Particles Modified with Gelatin as Novel Small Carriers for Mammalian Cells
- Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil
- Effect of Hydrostatic Pressure on Hybridoma Cell Metabolism
- Effect of Temperature on Cell Population Balance in Dexter's Culture of Murine Bone Marrow Hematopoietic Cells with Stromal Cells
- Selective Retension of Active Cells Employing Low Centrifugal Force at the Medium Change during Suspension Culture of Chinese Hamster Ovary Cells Producing tPA
- Acetone-Butanol Fermentation by Clostridium aurantibutyricum ATCC 17777 from a Model Medium for Palm Oil Mill Efflluent
- Knowledge-Based Design and Operation of Bioprocess Systems
- Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells.
- Effect of Concentrations of Murine Stromal and Hematopoietic Cells on the Progenitors Expansion in Their Three-Dimensional Coculture in Nonwoven Fabrics
- HPLC Monitoring System for Lactic Acid Fermentation
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Xanthan Gun Production in a Fermentor with Twin Impellers