Effect of αl, 2-Mannosidic Linkage Located in α1, 3-Branch of Man6GlcNAc2 Oligosaccharide on Enzyme Activity of Recombinant Human Man_9-Mannosidase Produced in Escherichia coli

スポンサーリンク

概要

• 論文の詳細を見る

• We produced the human kidney Man_9-mannosidase in Escherichia coli and studied the effect of the α-1, 2-mannosidic linkage located in the α branch of Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, an N-linked oligosaccharide, on the enzyme activity. The α1, 2-mannose residue influenced the rate of hydrolysis of and substrate preference for other α-1, 2-mannosidic linkages.

• 社団法人日本生物工学会の論文
• 2001-04-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• Fujiyama Kazuhito

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  The International Center for Biotechnology, Osaka University

• Yoshida Toshiomi

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• MORAN DANIEL

  The International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Moran Daniel

  The International Center For Biotechnology Osaka University:(present Address)kobe Technical Center P

• Moran Daniel

  大阪大学生物工学国際交流センター

• Moran Daniel

  The International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  The International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• SAKURADANI SHUJI

  The International Center for Biotechnology, Osaka University

• Yoshida T

  Kinki Univ. Nara Jpn

• Fujiyama Kazuhito

  The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn

• Sakuradani Shuji

  The International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大 工

関連論文

• Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum(PLANT BIOTECHNOLOGY)
• The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants(PLANT BIOTECHNOLOGY)
• The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants
• Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
• Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
• 611 2-ビドロキシ-4-メチルチオ酪酸アミド生産菌のニトリルヒドラターゼ遺伝子のクローニングと解析(酵素・酵素工学・タンパク質工学,一般講演)
• 923 NHaseを用いた2-ヒドロキシ-4-メチルチオ酪酸アミドの生産
• Glycoproteins Secreted from Suspension-cultured Tobacco BY2 Cells have Distinct Glycan Structures from Intracellular Glycoproteins(Biochemistry & Molecular Biology)
• Identification of Putative Gene Encoded on ORF16 of the 81 kb Contig of Arabidopsis thaliana Chromosome III as α-Mannosidase
• Structures of N-Linked Oligosaccharides of Glycoproteins from Tobacco BY2 Suspension Cultured Cells
• 1330 Structures of N-linked Glycoproteins from Tobacco BY2 Suspension Cultured Cells
• 2A16-4 epsilon-Poly-L-lysine(PL)分解酵素遺伝子の取得と破壊株を用いた機能解析(遺伝子工学・核酸工学,一般講演)
• Cloning and characterization of a β-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System(PLANT BIOTECHNOLOGY)
• N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice
• Human N-Acetylglucosaminyltransferase I. : Expression in Escherichia coli as a Soluble Enzyme, and Application as an Immobilized Enzyme for the Chemoenzymatic Synthesis of N-Linked Oligosaccharides
• Construction of Xylose-Assimilating Saccharomyces cerevisiae
• 181. Expression of Escherichia coli Transhydrogenase Gene in Saccharomyces cerevisiae
• 103 Expression of Xylose Reductase and Xylitol Dehydrogenase Genes from Pichia stipitis in Saccharomyces cerevisiae
• 564 Expression of Xylose Reductase of ___- ___- in ___- ___-.
• Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
• Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
• Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
• グリーンバイオプロセスの開発 : アクリルアミド工業化研究から学ぶ点
• 570 天然資源ロジンの微生物変換(酵素・酵素工学・タンパク質工学,一般講演)
• Divergent Structures of Carbazole Degradative car Operons Isolated from Gram-negative Bacteria
• Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively(Microbiology & Fermentation Technology)
• Expression, Purification, and Characterization of 2'-Aminobiphenyl-2,3-diol 1,2-dioxygenase from Carbazole-degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Purification and Characterization of meta-Cleavage Compound Hydrolase from a Carbazole Degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Organization and Transcriptional Characterization of Catechol Degradation Genes Involved in Carbazole Degradation by Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• New Classification System for Oxygenase Components Involved in Ring-Hydroxylating Oxygenations
• 3P-1072 エナンチオ選択的イミン還元酵素の探索と特徴解析(2a酵素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
• Purification, Characterization and Gene Cloning of Thermostable Ο-Acetyl-L-Homoserine Sulfhydrylase Forming γ-Cyano-α-Aminobutyric Acid (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Purification, Characterization and Gene Cloning of Thermostable ο-Acetyl-L-Serine Sulfhydrylase Forming β-Cyano-L-Alanine
• Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
• Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
• Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
• Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
• Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein
• Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-Directed Mutagenesis
• 2E09-3 炭酸固定反応を触媒する3,4-ジヒドロキシ安息香酸脱炭酸酵素の反応特性と一次構造解析(生物化学工学,一般講演)
• 3A10-1 炭酸固定反応を触媒する4-ヒドロキシ安息香酸脱炭酸酵素の反応特性と一次構造解析(酵素学・酵素工学・タンパク質工学,一般講演)
• 1A15-3 微生物触媒による 2,6-ジメチルピリジンのメチル基酸化反応
• 569 微生物触媒による芳香族カルボン酸のアルコールへの還元(酵素・酵素工学・タンパク質工学,一般講演)
• Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
• Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
• Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana(PLANT BIOTECHNOLOGY)
• Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells(PLANT BIOTECHNOLOGY)
• N-Linked Glycan Structures of a Mouse Monoclonal Antibody Produced from Tobacco BY2 Suspension-Cultured Cells(CELL AND TISSUE ENGINEERING)
• Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Effect of αl, 2-Mannosidic Linkage Located in α1, 3-Branch of Man6GlcNAc2 Oligosaccharide on Enzyme Activity of Recombinant Human Man_9-Mannosidase Produced in Escherichia coli
• Characterization of Recombinant Human Man_9-Mannosidase Expressed in Escherichia coli
• A Mass Spectrometry Membrane Probe and Practical Problems Associated with Its Application in Fermentation Processes
• On-Line Monitoring and Control of Aceotone-Butanol Fermentation by Membrane-Sensor Mass Spectrometry
• 1E14-4 Alcaligenes sp. IFO14130の菊酸エチルエステルと6-アミノヘキサン酸環状ダイマーに作用する加水分解酵素の関係(生物化学工学,一般講演)
• Cloning of cDNA and Characterization of Anti-RNase A Monoclonal Antibody 3A21
• 519 Control of Acetic Acid Accumulation during Cultivation of Recombinant E__-. coli AT2471.
• Characteristic of the Oligosaccharide Structure of Jack Bean α-Mannosidase with Some Lectins
• Enhancement of Recombinant Soluble Dengue Virus 2 Envelope Domain III Protein Production in Escherichia coli trxB and gor Double Mutant(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of Monascus Pigments by a Solid-Liquid State Culture Method
• Physiologically Motivated Strategies for Control of the Fed-Batch Cultivation of Recombinant Escherichia coli for Phenylalanine Production
• 921 炭酸固定反応をも触媒する脱炭酸酵素の探索 : インドール-3-カルボン酸脱炭酸酵素の発見
• Interactive Dual Control of Glucose and Glutamine Feeding in Hybridoma Cultivation
• 1E14-3 アミダーゼが触媒するアミドの生成反応(生物化学工学,一般講演)
• Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
• Synthesis and Evaluation of Novel Peptide-Lipid
• Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
• Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
• Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
• Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
• 1116 Dye-metal affinity chromatography in mixed mode for purification of alcohol dehydrogenase
• 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
• 1E13-4 2-ブチル-1,3-プロパンジオールの微生物交換(生物化学工学,一般講演)
• Novel Reversible Indole-3-carboxylate Decarboxylase Catalyzing Nonoxidative Decarboxylation(Microbiology & Fermentation Technology)
• N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells
• Continuous Ethanol Production by a Concentrated Culture of Flocculating Yeast
• Cloning of cDNAs Encoding Two Peroxidases of Arabidopsis thaliana and Their Organ-Specific Expression
• Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells
• Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation
• Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
• Phenylalanine Production by Periodic Induction of Gene Expression Using a Temperature-Distributed Dual Fermenter System
• Taxonomic characterization of Shiraia-like fungi isolated from bamboos in Japan
• Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N′-Acetylchitobiosyl Unit
• Enhancement of Anthocyanin Production by Perilla frutescens Cells in a Stirred Bioreactor with Internal Light Irradiation
• The Phylogeny of the Cactophilic Yeasts Based on the 18S Ribosomal RNA Gene Sequences : The Proposals of Phaffomyces antillensis and Starmera caribaea, New Combinations
• Production and Characterization of Active Soluble Human β1, 4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal β1⟶4 GlcNAc Linkage
• Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans : Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation
• Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
• Molecular Cloning and Expression of Two Novel β-N-Acetylglucosaminidases from Silkworm Bombyx mori
• Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
• Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells(PLANT BIOTECHNOLOGY)
• Carbonic Anhydrase in Chlamydomonas reinhardtii I. : Localization
• Improved expression and characterization of recombinant human Golgi α1, 2-mannosidase I isoforms (IA2 and IC) by Escherichia coli(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana : Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N'-Acetylchitobiosyl Unit
• N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells(PLANT BIOTECHNOLOGY)
• Arabidopsis β1,2-xylosyltransferase : Substrate specificity and participation in the plant-specific N-glycosylation pathway(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells
• 3Ha11 Application of different 5' untranslated sequences for higher expression of human β-glucocerebrosidase in tobacco BY2 suspension culture
• Changes in Color Parameters during Fermentation and Storage of Red Wine Using Thai Roselle under Different pHs and SO2 Concentrations.

もっと見る 閉じる

スポンサーリンク