Enhancement of Anthocyanin Production by Perilla frutescens Cells in a Stirred Bioreactor with Internal Light Irradiation

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概要

• 論文の詳細を見る

• Oxygen supply and light irradiation exhibited significant influence on the production of anthocyanin (red pigments) by suspended cultures of Perilla frutescens cells in a 2.6-l aerated and agitated bioreactor with a six-flat-bladed turbine. When the initial volumetric oxygen tranfer coefficient (k_Lα) value was below 10 h^<-1> and light was not irradiated, the anthocyanin production was never over 0.6 g/l. By modification of a gas sparger, the oxygen supply capability of the bioreactor was remarkably improved, and 1.65 g/l of anthocyanin was obtained at an enhanced k_Lα value of 15.4 h^<-1>. Moreover, it was found that anthocyanin accumulation at a 0.2 vvm aeration rate was higher than that at 0.1 or 0.4 vvm in the modified bioreactor, with the other cultivation conditions kept the same. Light irradiation also significantly increased anthocyanin accumulation in the stirred reactor at a low k_Lα value, i.e. 9.9 h^<-1>. However, a combination of irradiation with a higher oxygen supply reduced the production of anthocyanin in the bioreactor.

• 社団法人日本生物工学会の論文
• 1993-04-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Zhong J‐j

  East China Univ. Sci. And Technol. Shanghai Chn

• Fujiyama Kazuhito

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• ZHONG JIAN-JIANG

  International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers

• YOSHIDA MASASHI

  International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers

• Zhong Jian-jiang

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Yoshida Masashi

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

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