N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice
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概要
- 論文の詳細を見る
Human lactoferrin was produced in genetically engineered rice. N-linked glycan structures of recombinant human lactoferrin were determined. The oligosaccharides liberated by hydrazinolysis were labeled with 2-aminopyridine (PA). The PA-labeled glycans were purified by reverse-phase and size-fractionation HPLCs. The structures of these glycans were identified by HPLC, exoglycosidase digestion, and matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry. The glycan structures determined were ManFucXylGlcNAc2 (3.4%), Man2FucGlcNAc2 (2.1%), Man3FucGlcNAc2 (2.5%), Man3FucXylGlcNAc2 (42.5%), two isomers of Man2FucXylGlcNAc2 (39.1%), Man3XylGlcNAc2 (6.5%), and Man2XylGlcNAc2 (3.9%).
- 社団法人 日本農芸化学会の論文
- 2004-12-23
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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YANAGIHARA Itaru
Department of Environmental Medicine, Osaka Medical Center and Research Institute for Maternal and C
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Yanagihara Itaru
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
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Honda Takeshi
Department of Bacterial Infections, Research Institute for Microbial Disease, Osaka University
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SAKAI Yohei
International Center for Biotechnology, Osaka University
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MISAKI Ryo
International Center for Biotechnology, Osaka University
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ANZAI Hiroyuki
Gene Research Center, Ibaraki University
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SEKI Tatsuji
International Center for Biotechnology, Osaka University
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Kazuhito Fujiyama
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
大阪大学生物工学国際交流センター
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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Fujiyama Kazuhito
Department Of Fermentation Technology Osaka University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka University
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Misaki Ryo
International Center For Biotechnology Osaka University
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Misaki Ryo
The International Center For Biotechnology Osaka University
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Seki Tatsuji
International Center For Biotechnology Osaka University
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Seki Tatsuji
大阪大学生物工学国際交流センター
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Honda T
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
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Honda T
Department Of Bacterial Infection Research Institute For Microbial Diseases Osaka University
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Anzai Hiroyuki
Gene Research Center Ibaraki University
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Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
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Sakai Yohei
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
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Yanagihara Itaru
Department Of Developmental Infectious Diseases Research Institute Osaka Medical Center For Maternal
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Honda Takeshi
Research Institute For Microbial Diseases Osaka University
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Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
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